Avian Influenza A H6N1 Nucleoprotein Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Avian Influenza A H6N1 Nucleoprotein Antibody [NB100-56572] - Staining of influenza-infected MDCK cells at 1:10 dilution. Image Courtesy of Catherine Thompson, The University of Reading

Immunohistochemistry: Avian Influenza A H6N1 Nucleoprotein Antibody [NB100-56572] - VN1203 infected & replicated in Purkinje cells, deep cerebellar nuclei of the cerebellum. Representative sections of cerebellum of ferrets instilled with allantoic fluid (A,B,G,H), HK483 (C,D,I,J), VN1203 (E,F,K,L). Viral antigen detected with IMGENEX 5187-A for avian influenza A NP & visualized with DAB. Brown stain indicates presence of virus. Counterstained with Luxol fast blue. (B-F) higher magnification of boxes in A-E respectively. H) White arrow indicates molecular layer, black arrow indicates granule layer, black arrowhead indicates a Purkinje cell, white arrowhead indicates Purkinje cell axons. L) Arrow indicate infected granule cell and arrowhead indicates infected Purkinje cell. Scale bars represent: A,C,E,G,I, - 2 mm; B,D,F,H,J,L - 300 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0046605) licensed under a CC-BY license.

Immunohistochemistry: Avian Influenza A H6N1 Nucleoprotein Antibody [NB100-56572] - VN1203 induced significant pathology despite similar nasal turbinate titers in ferrets infected with either virus. Viral titers of homogenized nasal turbinates were graphed as mean +/- SEM for VN1203 or HK483 infected ferrets. D-I. Representative samples of nasal turbinate tissue of ferrets instilled with allantoic fluid (D,G), HK483 (E,H), or VN1203 (F,I). D-F were stained with H&E, G-I were stained with anti-avian influenza NP and counterstained with Luxol fast blue. F. # indicates inflammatory cells infiltrating the underlying stroma. H,I. Arrows point to H5N1 infected cells. Scale bars: D-F, 300 um; G-I, 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0046605) licensed under a CC-BY license.

Immunohistochemistry: Avian Influenza A H6N1 Nucleoprotein Antibody [NB100-56572] - VN1203 infection in the ferret brain was multifocal and evident in the olfactory tract.Representative sections from the frontal lobe of ferrets intranasally instilled with allantoic fluid (A,D), on: olfactory nucleus, rf: rhinal fissure, HK483 (B,E) or VN1203 (C,F) were stained for viral antigen with IMGENEX 5187-A for avian influenza A NP and visualized with DAB. Brown stain indicates the presence of virus. Counterstained with Luxol fast blue. Scale bars: A-C) 5 mm, D-F) 300 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0046605) licensed under a CC-BY license.

• Reactivity: V-Vi
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Avian Influenza A H6N1 Nucleoprotein Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to amino acids 428-441 (AFTGNTEGRTSDMR) of avian flu nucleoprotein were used as the immunogen, GenBank no ADC34563.1.
• Specificity: The amino acid sequence used as immunogen has 100% identity to the H5N1 virus in chicken, duck and swine.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.1-1 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Thompson et al (2006))
• Application Notes: This failed by western blot on the recombinant fusion protein.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Avian Influenza A H6N1 Nucleoprotein Antibody - BSA Free

• NP
• Nucleoprotein

Background

An H6N1 influenza virus was isolated from a green-winged teal during the H5N1 outbreak in Hong Kong Special Administrative Region (SAR) in 1997. This virus possesses similar genes encoding internal proteins as in the human H5N1 and H9N2 influenza viruses. In 1999, influenza viruses from quail infected two humans in Hong Kong. suggesting the potential for avian influenza viruses to cross the species barrier and infect humans without prior reassortment in an intermediate host, such as the pig. The common features shared by H5N1 and H9N2 influenza viruses isolated from humans are the genes encoding the proteins of the replicating complex, the matrix protein (M) gene, the nonstructural protein (NS) gene, nucleoprotein (NP), and N1 neuraminidase (NA), This virus essentially represents the reemergence of the H5N1 influenza viruses with a different hemagglutinin (HA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Avian Influenza A H6N1 Nucleoprotein Antibody (NB100-56572)(3)

Publications using NB100-56572

• Plourde JR, Pyles JA, Layton RC et al. Neurovirulence of H5N1 infection in ferrets is mediated by multifocal replication in distinct permissive neuronal cell regions. PLoS One. 2012-01-01 [PMID: 23056366]
• Mitchell H, Levin D, Forrest S et al. Higher level of replication efficiency of 2009 (H1N1) pandemic influenza virus than those of seasonal and avian strains: kinetics from epithelial cell culture and computational modeling. J Virol. 2011-01-01 [PMID: 21068247]
• Thompson CI, Barclay WS, Zambon MC, Pickles RJ. Infection of human airway epithelium by human and avian strains of influenza a virus. J Virol. 2006-08-01 [PMID: 16873262] (IHC-P)