ASB8 Antibody (OTI3A12) - Azide and BSA Free

Western Blot: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASB8 .

Immunohistochemistry: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)

Immunohistochemistry: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ASB8 Antibody (OTI3A12) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ASB8 (NP_077000) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASB8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 31.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ASB8 Antibody (OTI3A12) - Azide and BSA Free

• ankyrin repeat and SOCS box containing 8
• ankyrin repeat and SOCS box protein 8
• ankyrin repeat and SOCS box-containing 8
• ASB-8
• FLJ21255
• MGC5540

Background

ASB8 (Ankyrin repeat and SOCS box protein 8) is a 288 amino acid protein that has its highest level of expression in skeletal muscle. It is also expressed in heart, brain, placenta, liver, kidney and pancreas. ASB8 contains a SOCS box domain suggesting that it may be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It also contains 4 ankyrin repeats which mediate protein-protein interactions and are among the most common structural motifs in known proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASB8