Anthrax PA Antibody - Azide and BSA Free

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Western Blot: Anthrax PA Antibody - Azide Free [NB100-55743] - Analysis of Anthrax PA in recombinant protein using this antibody (Lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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Anthrax PA Antibody - Azide and BSA Free Summary

Description
Novus Biologicals Rabbit Anthrax PA Antibody - Azide and BSA Free (NB100-56670) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 186-192 of the Anthrax PA protein (approx. 83 kDa).
Specificity
PA is cleaved from the 83 kDa form to the 63 kDa form at amino acids 164-167. Thus the antibody (made against amino acids 186-192) should recognize both the 83 kDa and 63 kDa PA forms, but not the 20 kDa cleavage fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000

Reactivity Notes

Anthrax (Bacillus anthracis) Protective Antigen (PA)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera with sterile - filtered PBS
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Anthrax PA Antibody - Azide and BSA Free

  • anthrax PA
  • Anthrax toxins translocating protein
  • Bacillus anthracis protective antigen
  • PA 83
  • PA
  • PA83
  • PAG
  • Paga
  • Protective antigen

Background

Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxic entry into cells. PA is secreted from Bacillus anthracis as an 83 kDa (PA83) protein which is cleaved by furin protease, releasing a 20 kDa fragment. The 63 kDa portion (PA63) of the cleavage product forms a heptamer, which binds the toxins LF and EF. This complex enters via receptor-mediated endocytosis where LF and EF exert their toxic effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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