Anthrax LF Antibody (5F502.2) - BSA Free

Western Blot: Anthrax LF Antibody (5F502.2) [NB100-56592] - analysis of Anthrax LF in recombinant protein using Anthrax LF antibody at 0.1 ug/ml.

• Reactivity: Hu
• Applications: WB
• Clone: 5F502.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Anthrax LF Antibody (5F502.2) - BSA Free Summary

• Description: Novus Biologicals Mouse Anthrax LF Antibody (5F502.2) - BSA Free (NB100-56592) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 779-792 of the Anthrax LF protein (approx. 90 kD).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1-1 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Anthrax LF Antibody (5F502.2) - BSA Free

• Anthrax lethal factor
• Anthrax lethal toxin endopeptidase component
• Anthrax LF
• B.anthracis lethal factor
• bacillus anthracis lethal factor
• Lef
• LF

Background

Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.