Anthrax LF Antibody (5F502.2) [HRP]

• Reactivity: Hu
• Applications: WB
• Clone: 5F502.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

Anthrax LF Antibody (5F502.2) [HRP] Summary

• Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 779-792 of the Anthrax LF protein (approx. 90 kD).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Anthrax LF Antibody (5F502.2) [HRP]

• Anthrax lethal factor
• Anthrax lethal toxin endopeptidase component
• Anthrax LF
• B.anthracis lethal factor
• bacillus anthracis lethal factor
• Lef
• LF

Background

Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.