Anthrax EF Antibody - BSA Free

• Reactivity: Ba
• Applications: ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Anthrax EF Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to 16 amino acids near the carboxy terminus of the Anthrax edema factor protein. The immunogen is located within the last 50 amino acids of Anthrax Edema Factor. Amino Acid Squence: NFTENETDNFEVFQK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Anthrax EF Antibody - BSA Free

• Adenylyl cyclase
• Anthrax edema toxin adenylate cyclase component
• ATP pyrophosphate-lyase
• Calmodulin-sensitive adenylate cyclase
• Edema factor 1 (EF 1)

Background

Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF) (1,2 for review). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) (3) and capillary morphogenesis protein 2 (CMG2) (4), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm (5). EF is a calmodulin and Ca++-dependent adenylate cyclase responsible for the edema seen in the disease. It is thought to benefit the B. anthracis bacteria by inhibiting cells of the host immune system (reviewed in 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.