Amyloid Fibrils Antibody - BSA Free

Western Blot: Amyloid Fibrils Antibody [NBP1-97929] - Western blot analysis of Human Abeta42 fibrils and prefibrillar oligomers showing detection of Amyloid Fibrils Fibrils (OC) protein using Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929). Primary Antibody: Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929) at 1:1000. Courtesy of: Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., et al. (2003). Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science 300, 486-489. doi: 10.1126/science.1079469.

Dot Blot: Amyloid Fibrils Antibody [NBP1-97929] - Dot blot analysis using Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929) at 1:500, 1:5000. Beta Amyloid Fibrils HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.

• Reactivity: Hu
• Applications: WB, DB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Amyloid Fibrils Antibody - BSA Free Summary

• Immunogen: Fibrils prepared from human amyloid beta 42 peptide
• Localization: Membrane
• Specificity: Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrillar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on species homology.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: A 1:1000 dilution of Amyloid Fibrils (OC) Antibody was sufficient for detection of amyloid fibrils on PVDF membranes using transferred fibrils by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Amyloid Fibrils Antibody (NBP1-97929)(4)

Publications using NBP1-97929

• Levites Y, Dammer EB, Ran Y et Al. Integrative proteomics identifies a conserved A? amyloid responsome, novel plaque proteins, and pathology modifiers in Alzheimer's disease Cell Rep Med 2024-08-09 [PMID: 39127040]
• Wei Z, Li D, Shi J Alterations of Spatial Memory and Gut Microbiota Composition in Alzheimer's Disease Triple-Transgenic Mice at 3, 6, and 9 Months of Age American journal of Alzheimer's disease and other dementias 2023-05-11 [PMID: 37169734] (IHC-P, Mouse)
  
  Details:
  Dilutions: 1:500
• Hurst C, Pugh DA, Abreha MH et al. Integrated Proteomics to Understand the Role of Neuritin (NRN1) as a Mediator of Cognitive Resilience to Alzheimer's Disease Molecular & cellular proteomics : MCP 2023-04-04 [PMID: 37024090] (WB, Human)
• Zhao Y, Qiao S, Zhang B Et al. A novel neuroinflammation-responsive hydrogel based on mimicking naked mole rat brain microenvironment retards neurovascular dysfunction and cognitive decline in Alzheimer s disease Front Aging Neurosci 2022-07-21 [PMID: 35860673] (Cytometric Bead Assay Standard)

Review for Amyloid Fibrils Antibody (NBP1-97929) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Sam Marsh IF Human 04/08/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Transgenic Mouse Brain (40um)
• Species: Human