![Western Blot: Amyloid Fibrils Antibody [NBP1-97929] - Western blot analysis of Human Abeta42 fibrils and prefibrillar oligomers showing detection of Amyloid Fibrils Fibrils (OC) protein using Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929). Primary Antibody: Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929) at 1:1000. Courtesy of: Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., et al. (2003). Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science 300, 486-489. doi: 10.1126/science.1079469.](http://images.novusbio.com/fullsize/Amyloid-Fibrils-Antibody-Western-Blot-NBP1-97929-img0008.jpg "Western Blot: Amyloid Fibrils Antibody [NBP1-97929] - Western blot analysis of Human Abeta42 fibrils and prefibrillar oligomers showing detection of Amyloid Fibrils Fibrils (OC) protein using Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929). Primary Antibody: Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929) at 1:1000. Courtesy of: Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., et al. (2003). Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science 300, 486-489. doi: 10.1126/science.1079469.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Amyloid Fibrils Antibody - BSA Free (NBP1-97929) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-Amyloid Fibrils Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Fibrils prepared from human amyloid beta 42 peptide |
| Localization | Membrane |
| Specificity | Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrillar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on species homology. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | A 1:1000 dilution of Amyloid Fibrils (OC) Antibody was sufficient for detection of amyloid fibrils on PVDF membranes using transferred fibrils by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein A purified |
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reviewed by:
Sam Marsh |
IF | Human | 04/08/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Dot Blot: Amyloid Fibrils Antibody [NBP1-97929] - Dot blot analysis using Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929) at 1:500, 1:5000. Beta Amyloid Fibrils HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.](http://images.novusbio.com/fullsize/Amyloid Fibrils Antibody-Dot Blot-NBP1-97929-img0009.jpg "Dot Blot: Amyloid Fibrils Antibody [NBP1-97929] - Dot blot analysis using Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929) at 1:500, 1:5000. Beta Amyloid Fibrils HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
