![Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/nbp3-20190_adipogenesis-antibody-pack-western-blot-352023131949.jpg "Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC |
| Description | This pack contains 1 vial each of: MAB6898-SP (0.025 mg), NB600-582SS (0.025 mg), MAB7094-SP (0.025 mg), AF3150-SP (0.025 mg), NB400-144SS (0.025 mg), NB110-40760SS (0.025 mL), NBP2-22106SS (0.025 ml), AF2989-SP (0.025 mg), HAF017 (1.0 ml), HAF008 (1.0 ml), and HAF007 (1.0 ml). |
| Immunogen | See individual datasheets. |
| Dilutions |
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| Application Notes | See individual datasheets of components for their validated applications |
| Storage | Storage is content dependent. |
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ADFP and Lipid Storage Disease Adipocyte differentiation-related protein (ADFP) is found on the surface of lipid droplets in most mammalian cell types. In mammals, lipid droplets are most abundant in adipose tissue, where stored triacylglycerol (TAG) provides the primary energy res... Read full blog post. |
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Appetite and Energy: A Ghrelin Balancing Act Ghrelin is the only potent orexigenic peptide in circulation. It stimulates food intake and leads to metabolism regulation, positive energy balance, adipogenesis, and body weight gain. However, in studies using ghrelin antibodies, the physiological si... Read full blog post. |
![Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western Blot: CD36 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD36 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20190_adipogenesis-antibody-pack-western-blot-352023132142.jpg "Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western Blot: CD36 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD36 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Flow Cytometry: Adipogenesis Antibody Pack [NBP3-20190] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/nbp3-20190_adipogenesis-antibody-pack-flow-cytometry-35202313258.jpg "Flow Cytometry: Adipogenesis Antibody Pack [NBP3-20190] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CREB at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/nbp3-20190_adipogenesis-antibody-pack-western-blot-352023142028..jpg "Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CREB at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")