ACTL7B Antibody

Western Blot: ACTL7B Antibody [NB100-57848] - (0.5ug/ml) staining of Rat Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ACTL7B Antibody Summary

• Description: Novus Biologicals Goat ACTL7B Antibody (NB100-57848) is a polyclonal antibody validated for use in WB and ELISA. Anti-ACTL7B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DELHVDYELPDGK corresponding to internal region according to NP_079547.1.
• Specificity: This antibody is not expected to cross-react with Actin-like 7A.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACTL7B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in rat testis lysates (calculated MW of 45.5 kDa band according to rat NP_001020588.1 and 45.6 kDa band according to mouse NP_079547.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACTL7B Antibody

• actin-like 7B
• actin-like 7-beta
• actin-like protein 7B
• actin-like-7-beta
• Tact1

Background

The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene (ACTL7B), and related gene, ACTL7A, are intronless, and are located approximately 4 kb apart in a head-to-head orientation within the familial dysautonomia candidate region on 9q31. Based on mutational analysis of the ACTL7B gene in patients with this disorder, it was concluded that it is unlikely to be involved in the pathogenesis of dysautonomia. Unlike ACTL7A, the ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACTL7B Antibody (NB100-57848)(1)

Publication using NB100-57848

• Chadwick BP, Mull J, Helbling LA et al. Cloning, mapping, and expression of two novel actin genes, actin-like-7A (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia candidate region on 9q31. Genomics 1999-06-15 [PMID: 10373328]

Bioinformatics

• Gene Symbol: ACTL7B
• Entrez:
  • 11471(Mouse)
  • 313183(Rat)
• Uniprot:
  • Q4QR76(Rat)