Acetylcholine Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: All-NA
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

Acetylcholine Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Synthetic acetylcholine conjugated to Keyhole limpet Haemocyanin
• Specificity: Rabbit anti acetylcholine polyclonal antibody recognizes the small cationic neurotransmitter acetylcholine, involved in the transmission of nerve impulses across synapses. The structure of acetylcholine is preserved across the animal kingdom and the Rabbit anti acetylcholine is expected to recognize acetylcholine from all species posessing this neurotransmitter.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted cross-reactivities: Rat, Human

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Acetylcholine Antibody [CoraFluor™ 1]

• [2 (acetyloxy)ethyl]trimethylammonium
• ACh

Background

The chemical compound Acetylcholine, often abbreviated as ACh, was the first neurotransmitter to be identified. It is a chemical transmitter in the central nervous system (CNS) as well as in the parasympathetic nervous system in many organisms including humans. When it binds to Acetylcholine receptors of muscle fibers, stimulates those fibers to contract. Acetylcholine is also used in the brain, where it tends to cause excitatory actions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.