Acetyl-Lysine Antibody (RM101)

Western Blot: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - Analysis of HeLa cells nontreated or treated with Trichostatin A (TSA), using NBP2-77397.

Immunocytochemistry/Immunofluorescence: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - Immunocytochemical staining of HeLa cells using anti-Acetyl-Lysine antibody (NBP2-77397, red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).

Immunoprecipitation: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - A431 cell lysates, using NBP2-77397, was blotted with anti-PTEN mouse monoclonal antibody.

Immunocytochemistry/ Immunofluorescence: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP2-77397] - Immunocytochemical staining of HeLa cells using Clone RM101 (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).

• Reactivity: All
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP
• Clone: RM101
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Acetyl-Lysine Antibody (RM101) Summary

• Description: Novus Biologicals Rabbit Acetyl-Lysine Antibody (RM101) (NBP2-77397) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Acetyl lysine -BSA
• Specificity: This antibody reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysine with other modification.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:100-1:500
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500 -1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Acetyl-Lysine Antibody (RM101)

• acetyl Lysine
• AC-LYS-OH
• Lysine
• N-acetyl lysine

Background

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.