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![Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of Acetyl-Lysine in HeLA cells.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Western-Blot-NB100-74339-img0004.jpg "Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of Acetyl-Lysine in HeLA cells.")
![Immunocytochemistry/Immunofluorescence: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of lysine acetylated proteins (green) in HeLa cells left untreated (left panels) or treated with 0.3uM Trichostatin A for 16 hours (right panels).](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Immunocytochemistry-Immunofluorescence-NB100-74339-img0007.jpg)
![Chromatin Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of chromatin proteins with Acetyl-Lysine was performed using cross-linked chromatin from 4-10^6 LNCaP cells. Immunoprecipitation was performed using the Magnetic ChIP kit with 10ug of an Acetyl-Lysine monoclonal antibody. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Chromatin-Immunoprecipitation-NB100-74339-img0008.jpg)
![Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of Acetyl-Lysine was performed by loading various amounts of acetylated BSA or non-acetylated BSA per well and 10ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Acetyl-Lysine monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Western-Blot-NB100-74339-img0002.jpg)
![Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of lysine acetylated proteins from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM of Trichostatin A (TSA) for 16 hours was performed by loading 50 ug of the indicated whole cell lysates per well and 10ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Acetyl-Lysine monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Western-Blot-NB100-74339-img0003.jpg)
![Dot Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Specific detection of lysine acetylated histones using an Acetyl-Lysine monoclonal antibody. Histone peptides containing 384 different modification combinations spotted in duplicate on a peptide array were used according to the manufacturer instructions. A schematic and table with representative results (positive detection in red, negative in blue) are shown. Result interpretation and quantification were performed using the manufacturer software. Chemiluminescent detection was performed.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Dot-Blot-NB100-74339-img0012.jpg)
![Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Immunoprecipitation of acetylated alpha-Tubulin was performed.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of an Acetyl-Lysine monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2000 for at least 1 hour.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Immunoprecipitation-NB100-74339-img0005.jpg)
![Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of acetylated Histone H3 (Lys9) was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of the indicated lysate with 3ug of an Acetyl-Lysine mAb overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hr. The membrane was probed with an Acetyl-Histone H3 (Lys9) mAb at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Immunoprecipitation-NB100-74339-img0006.jpg)
![Dot Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Specific detection of lysine acetylated histones using an Acetyl-Lysine monoclonal antibody. Histone peptides containing 384 different modification combinations spotted in duplicate on a peptide array were used according to the manufacturer's instructions (Active Motif). Representative results showing specific detection of acetylated Histone H4 K20 (blue circles, panel A) and H3 K9 (blue circles, panel B). Note that Histone H4 K20me2/K20me3 (red and yellow circles, panel A) and H3 K9me2/K9m3 (red and yellow circles, panel B) are not detected by the Acetyl-Lysine monoclonal antibody.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-1C6-Dot-Blot-NB100-74339-img0013.jpg)
![Western Blot: Acetyl-Lysine Antibody (7F8) [NB110-96876] - Analysis of Acetyl-Lysine showing detection of Acetyl-Lysine protein using Mouse Anti-Acetyl-Lysine Monoclonal Antibody, Clone 7F8 . Primary Antibody: Mouse Anti-Acetyl-Lysine Monoclonal Antibody at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.](http://images.novusbio.com/fullsize/Acetyl-Lysine-Antibody-7F8-Western-Blot-NB110-96876-img0005.jpg "Western Blot: Acetyl-Lysine Antibody (7F8) [NB110-96876] - Analysis of Acetyl-Lysine showing detection of Acetyl-Lysine protein using Mouse Anti-Acetyl-Lysine Monoclonal Antibody, Clone 7F8 . Primary Antibody: Mouse Anti-Acetyl-Lysine Monoclonal Antibody at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.")
![Immunohistochemistry: Acetyl-Lysine Antibody (7F8) [NB110-96876] - Immunohistochemistry analysis using Mouse Anti-Acetyl-LysineMonoclonal Antibody, Clone 7F8 (NB110-96876). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetyl-LysineMonoclonal Antibody (NB110-96876) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.](http://images.novusbio.com/fullsize/Acetyl-Lysine-Antibody-7F8-Immunohistochemistry-NB110-96876-img0006.jpg)
![Western Blot: Mouse Monoclonal Acetyl-Lysine Antibody (7F8) [NB110-96876] - Analysis using mouse liver lysates. Antibody concentration used was 1:1000, diluted in 1x TBST. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb110-96876_mouse-monoclonal-acetyl-lysine-antibody-7f8-western-blot-1612202493544..jpg)
![Immunoprecipitation: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] - Immunoprecipitation of A431 cell lysates, using Clone RM101 at 1/500 dilution, was blotted with anti-PTEN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/nbp3-25979_rabbit-acetyl-lysine-mab-rm101-azide-and-bsa-free-22620242311056.jpg "Immunoprecipitation: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] - Immunoprecipitation of A431 cell lysates, using Clone RM101 at 1/500 dilution, was blotted with anti-PTEN mouse monoclonal antibody.")
![Western Blot: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] - Western blot of HeLa cells nontreated or treated with Trichostatin A (TSA), using Clone RM101 at 1/2000 dilution.](http://images.novusbio.com/fullsize/nbp3-25979_rabbit-acetyl-lysine-mab-rm101-azide-and-bsa-free-22620242217449.jpg)
![Multiplex Immunoassay: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] - Clone RM101 recognizes acetylated lysine in peptides with different sequences. It does not react with unmodified lysine.](http://images.novusbio.com/fullsize/nbp3-25979_rabbit-acetyl-lysine-mab-rm101-azide-and-bsa-free-22620242311054.png)
![Immunocytochemistry/ Immunofluorescence: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] - Immunocytochemical staining of HeLa cells using Clone RM101 (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-25979_rabbit-acetyl-lysine-mab-rm101-azide-and-bsa-free-24620241332465.png)
![Western Blot: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - Analysis of HeLa cells nontreated or treated with Trichostatin A (TSA), using NBP2-77397.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-RM101-Western-Blot-NBP2-77397-img0002.jpg "Western Blot: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - Analysis of HeLa cells nontreated or treated with Trichostatin A (TSA), using NBP2-77397.")
![Immunocytochemistry/Immunofluorescence: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - Immunocytochemical staining of HeLa cells using anti-Acetyl-Lysine antibody (NBP2-77397, red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-RM101-Immunocytochemistry-Immunofluorescence-NBP2-77397-img0001.jpg)
![Immunoprecipitation: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - A431 cell lysates, using NBP2-77397, was blotted with anti-PTEN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Acetylated-Lysine-Antibody-RM101-Immunoprecipitation-NBP2-77397-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP2-77397] - Immunocytochemical staining of HeLa cells using Clone RM101 (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-77397_rabbit-monoclonal-acetyl-lysine-antibody-rm101-immunocytochemistry-immunofluorescence-18920252338173.png)
![Western Blot: Acetyl-Lysine Antibody (3P8N3) [NBP3-15483] - Western blot analysis of extracts of various cell lines, using Acetyl-Lysine antibody (NBP3-15483) at 1:1000 dilution.HeLa NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37C for 18 hours. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Acetyl-Lysine-Antibody-3P8N3-Western-Blot-NBP3-15483-img0001.jpg "Western Blot: Acetyl-Lysine Antibody (3P8N3) [NBP3-15483] - Western blot analysis of extracts of various cell lines, using Acetyl-Lysine antibody (NBP3-15483) at 1:1000 dilution.HeLa NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37C for 18 hours. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")