![Immunocytochemistry/Immunofluorescence: 5-Carboxylcytosine Antibody [NBP2-59148] - 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the antibody against 5-caC , diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining.](http://images.novusbio.com/fullsize/5-Carboxylcytosine-Antibody-Immunofluorescence-NBP2-59148-img0001.jpg "Immunocytochemistry/Immunofluorescence: 5-Carboxylcytosine Antibody [NBP2-59148] - 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the antibody against 5-caC , diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining.")
| Reactivity | Hu, Mu, All-NASpecies Glossary |
| Applications | DB, ICC/IF, IP, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit 5-Carboxylcytosine Antibody - BSA Free (NBP2-59148) is a polyclonal antibody validated for use in ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The exact immunogen is propietary information. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: 5-Carboxylcytosine Antibody [NBP2-59148] - Immunoprecipitation was performed with the antibody against 5-caC on 2 ug of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP'd DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control.](http://images.novusbio.com/fullsize/5-Carboxylcytosine-Antibody-Immunoprecipitation-NBP2-59148-img0002.jpg "Immunoprecipitation: 5-Carboxylcytosine Antibody [NBP2-59148] - Immunoprecipitation was performed with the antibody against 5-caC on 2 ug of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP'd DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control.")
![Dot Blot: 5-Carboxylcytosine Antibody [NBP2-59148] - Analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo's were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure shows a high specificity of the antibody for the carboxylated cytosine.](http://images.novusbio.com/fullsize/5-Carboxylcytosine-Antibody-Dot-Blot-NBP2-59148-img0003.jpg "Dot Blot: 5-Carboxylcytosine Antibody [NBP2-59148] - Analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo's were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure shows a high specificity of the antibody for the carboxylated cytosine.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")