4-Hydroxynonenal Antibody (198960) [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4‑Hydroxynonenal ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for ...read more

Product Details

Summary
Reactivity MultiSpecies Glossary
Applications WB, Simple Western
Clone
198960
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

4-Hydroxynonenal Antibody (198960) [Unconjugated] Summary

Immunogen
KLH-coupled 4-Hydroxynonenal
Specificity
Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 3
using
MAB3249 in the following applications:

Publications
Read Publications using
MAB3249 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-Hydroxynonenal Antibody (198960) [Unconjugated]

  • 4 HNE
  • 4Hydroxynonenal
  • 4-Hydroxynonenal
  • HNE

Background

4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4-Hydroxynonenal Antibody (MAB3249)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 3 applications: ICC, IHC Not Specified, WB.


Filter By Application
ICC
(2)
IHC Not Specified
(1)
WB
(13)
All Applications
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Human
(2)
Mouse
(8)
Rat
(5)
Drosophila
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using MAB3249 Applications Species
JM Gemechu, A Sharma, D Yu, Y Xie, OM Merkel, A Moszczynsk Characterization of Dopaminergic System in the Striatum of Young Adult Park2-/- Knockout Rats Sci Rep, 2018;8(1):1517. 2018 [PMID: 29367643] (WB,, Rat) WB Rat
J Zhao, Z Tian, T Kadomatsu, P Xie, K Miyata, T Sugizaki, M Endo, S Zhu, H Fan, H Horiguchi, J Morinaga, K Terada, T Yoshizawa, K Yamagata, Y Oike Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29191837] (WB,, Mouse) WB Mouse
WS Hambright, RS Fonseca, L Chen, R Na, Q Ran Ablation of ferroptosis regulator glutathione peroxidase 4 in forebrain neurons promotes cognitive impairment and neurodegeneration Redox Biol, 2017;12(0):8-17. 2017 [PMID: 28212525] (WB,, Mouse) WB Mouse
J Shin, SJ Yang, Y Lim Gamma-tocopherol supplementation ameliorated hyper-inflammatory response during the early cutaneous wound healing in alloxan-induced diabetic mice Exp. Biol. Med. (Maywood), 2017;242(5):505-515. 2017 [PMID: 28211759] (WB,, Mouse) WB Mouse
J Seo, NN Singh, EW Ottesen, S Sivanesan, M Shishimoro, RN Singh Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene PLoS ONE, 2016;11(4):e0154390. 2016 [PMID: 27111068] (WB,, Mouse) WB Mouse
Garate I, Garcia-Bueno B, Madrigal J, Caso J, Alou L, Gomez-Lus M, Leza J Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress. J Neuroinflammation, 2014;11(0):8. 2014 [PMID: 24410883] (WB,, Rat) WB Rat
Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies. Am J Physiol Endocrinol Metab, 2014;306(4):E443-56. 2014 [PMID: 24368670] (WB,, Rat) WB Rat
Bufalino, Mary Ros, DeVeale, Brian, van der Kooy, Derek The asymmetric segregation of damaged proteins is stem cell-type dependent. J Cell Biol, 2013;201(4):523-30. 2013 [PMID: 23649805] (IHC Not Specified,, Drosophila) IHC Not Specified Drosophila
Margaritis M, Antonopoulos A, Digby J, Lee R, Reilly S, Coutinho P, Shirodaria C, Sayeed R, Petrou M, De Silva R, Jalilzadeh S, Demosthenous M, Bakogiannis C, Tousoulis D, Stefanadis C, Choudhury R, Casadei B, Channon K, Antoniades C Interactions between vascular wall and perivascular adipose tissue reveal novel roles for adiponectin in the regulation of endothelial nitric oxide synthase function in human vessels. Circulation, 2013;127(22):2209-21. 2013 [PMID: 23625959] (WB,, Human) WB Human
Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease. J Neuroinflammation, 2012;9(0):241. 2012 [PMID: 23092448] (WB,, Mouse) WB Mouse
Show All 16 Publications.

Review for 4-Hydroxynonenal Antibody (MAB3249) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB3249:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 02/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21429624
SpeciesMouse

Blocking

Blocking DetailsSee PMID 21429624

Details

Detection NotesSee PMID 21429624

Comments

CommentsIndependent review from antibodyresource.com

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Anonymous
02/19/2015
Application: WB
Species: Mouse