4-Hydroxynonenal Antibody (198960) [Alexa Fluor® 750]

• Applications: WB, IHC
• Clone: 198960
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

4-Hydroxynonenal Antibody (198960) [Alexa Fluor® 750] Summary

• Immunogen: KLH-coupled 4-Hydroxynonenal
• Specificity: Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.