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![Western Blot: Mouse Heart Whole Tissue Lysate (Adult Normal) [NB820-59660] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse heart lysate [NB820-59660].](http://images.novusbio.com/fullsize/Mouse-Heart-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59660-img0004.jpg "Western Blot: Mouse Heart Whole Tissue Lysate (Adult Normal) [NB820-59660] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse heart lysate [NB820-59660].")
![SDS-Page: Mouse Heart Whole Tissue Lysate (Adult Normal) [NB820-59660] - Ponceau S staining of whole normal mouse heart lysate using NB820-59660.](http://images.novusbio.com/fullsize/Mouse-Heart-Whole-Tissue-Lysate-Adult-Normal-SDS-Page-NB820-59660-img0005.jpg)
![Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Detection of alpha tubulin in lane 1. CHO/K1 Whole Cell Lysate (10 g) was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, primary antibody was used at 1:1000 overnight at 4C. Anti-Rabbit IgG (H&L) (GOAT) antibody IRDye800CW secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin.](http://images.novusbio.com/fullsize/CHO-K1-Whole-Cell-Lysate-Western-Blot-NB800-PC13-img0007.jpg "Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Detection of alpha tubulin in lane 1. CHO/K1 Whole Cell Lysate (10 g) was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, primary antibody was used at 1:1000 overnight at 4C. Anti-Rabbit IgG (H&L) (GOAT) antibody IRDye800CW secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin.")
![Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Coomassie stained SDS-PAGE of 35 ul of CHO Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 2). Molecular weight standards are shown in lane 1.](http://images.novusbio.com/fullsize/CHO-K1-Whole-Cell-Lysate-Western-Blot-NB800-PC13-img0004.jpg)
![SDS-Page: MDA-MB-231 Whole Cell Lysate [NBP3-18060] - SDS PAGE Results of MDA-MB-231 Whole Cell Lysate. Lane 1: MDA-MB-231 Whole Cell Lysate Reduced [10ug]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: MDA-MB-231 Whole Cell Lysate Non-Reduced [10ug]. 4-20% Gel, Coomassie Stained.](http://images.novusbio.com/fullsize/MDA-MB-231-Whole-Cell-Lysate-SDS-Page-NBP3-18060-img0001.jpg "SDS-Page: MDA-MB-231 Whole Cell Lysate [NBP3-18060] - SDS PAGE Results of MDA-MB-231 Whole Cell Lysate. Lane 1: MDA-MB-231 Whole Cell Lysate Reduced [10ug]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: MDA-MB-231 Whole Cell Lysate Non-Reduced [10ug]. 4-20% Gel, Coomassie Stained.")
![Western Blot: U-251 Whole Cell Lysate [NBP3-06998] - Western Blot results of Rabbit Anti-Beclin 1 Antibody using U-251 Whole Cell lysate.Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: U251-MG whole cell lysate (35ug) [+]. Primary Antibody: Anti-Beclin1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 60min at RT. Block: 5% BLOTTO/TBST.Expected MW: ~51kDa. Observed MW: ~53kDa.](http://images.novusbio.com/fullsize/U-251-Whole-Cell-Lysate-Western-Blot-NBP3-06998-img0002.jpg "Western Blot: U-251 Whole Cell Lysate [NBP3-06998] - Western Blot results of Rabbit Anti-Beclin 1 Antibody using U-251 Whole Cell lysate.Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: U251-MG whole cell lysate (35ug) [+]. Primary Antibody: Anti-Beclin1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 60min at RT. Block: 5% BLOTTO/TBST.Expected MW: ~51kDa. Observed MW: ~53kDa.")
![SDS-Page: U-251 Whole Cell Lysate [NBP3-06998] - SDS-PAGE Results of U-251 Whole Cell Lysate. Lane 1: U-251 Whole Cell lysate(10ug) Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: U-251 Whole Cell lysate(10ug) Non-Reduced. Results show wide range of molecular weight bands with no sign of degradation.](http://images.novusbio.com/fullsize/U-251-Whole-Cell-Lysate-SDS-Page-NBP3-06998-img0001.jpg)
![SDS-Page: Rat Brain Whole Tissue Lysate (Post-Natal Whole) [NBP3-05287] - Lane 1: Rat Brain, Post Natal, WCL (10ug) Reduced. Lane 2: Rat Brain, Post Natal, WCL (5ug) Reduced. Lane 3: Rat Brain, Post Natal, WCL (1ug) Reduced. Lane 4: Opal pre-stained Molecular Weight Markers. Lane 5: Rat Brain, Post Natal, WCL (1ug) Non-Reduced. Lane 6: Rat Brain, Post Natal, WCL (5ug) Non-Reduced. Lane 7: Rat Brain, Post Natal, WCL (10ug) Non-Reduced. Coomassie Blue stained. Observed and Expected MW: Various bands between ~11-245 kDa.](http://images.novusbio.com/fullsize/Rat-Brain-Whole-Tissue-Lysate-Post-Natal-Whole-SDS-Page-NBP3-05287-img0001.jpg "SDS-Page: Rat Brain Whole Tissue Lysate (Post-Natal Whole) [NBP3-05287] - Lane 1: Rat Brain, Post Natal, WCL (10ug) Reduced. Lane 2: Rat Brain, Post Natal, WCL (5ug) Reduced. Lane 3: Rat Brain, Post Natal, WCL (1ug) Reduced. Lane 4: Opal pre-stained Molecular Weight Markers. Lane 5: Rat Brain, Post Natal, WCL (1ug) Non-Reduced. Lane 6: Rat Brain, Post Natal, WCL (5ug) Non-Reduced. Lane 7: Rat Brain, Post Natal, WCL (10ug) Non-Reduced. Coomassie Blue stained. Observed and Expected MW: Various bands between ~11-245 kDa.")
![Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ERK2-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18632-img0001.jpg "Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.](http://images.novusbio.com/fullsize/Raji-Whole-Cell-Lysate-Western-Blot-NB820-59466-img0002.jpg "Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.")
![SDS-Page: Raji Whole Cell Lysate [NB820-59466] - Protein lysate shows consistent pattern.](http://images.novusbio.com/fullsize/Raji-Whole-Cell-Lysate-SDS-Page-NB820-59466-img0001.jpg)
![Western Blot: Mouse Kidney Whole Tissue Lysate (Adult Normal) [NB820-59661] - Western blot analysis of Actin (NB600-532) using NB820-59961.](http://images.novusbio.com/fullsize/Mouse-Kidney-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59661-img0004.jpg "Western Blot: Mouse Kidney Whole Tissue Lysate (Adult Normal) [NB820-59661] - Western blot analysis of Actin (NB600-532) using NB820-59961.")
![SDS-Page: Mouse Kidney Whole Tissue Lysate (Adult Normal) [NB820-59661] - Ponceau S staining of whole normal mouse kidney lysate using NB820-59661.](http://images.novusbio.com/fullsize/Mouse-Kidney-Whole-Tissue-Lysate-Adult-Normal-SDS-Page-NB820-59661-img0005.jpg)