CHO K1 Whole Cell Lysate

Images

 
Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Detection of alpha tubulin in lane 1. CHO/K1 Whole Cell Lysate (10 g) was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% ...read more
Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Coomassie stained SDS-PAGE of 35 ul of CHO Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 2). Molecular weight ...read more

Product Details

Summary
Reactivity ChHaSpecies Glossary
Applications WB
Concentration
1 mg/ml

Order Details

CHO K1 Whole Cell Lysate Summary

Specificity
Cell Line: Chinese Hamster Ovary (CHO)

Applications/Dilutions

Application Notes
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer [62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8], 10% (v/v) Glycerol
Preservative
No Preservative
Concentration
1 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Reproductive
Subcellular Fraction
Whole

Background

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

NB800-PC13

Additional CHO K1 Products

Array NB800-PC13

Blogs on CHO K1

There are no specific blogs for CHO K1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

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