VSIG4 Antibodies

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kuppfer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kuppfer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="VSIG4 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse VSIG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4674) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kuppfer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human VSIG4. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB4646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

MAGH105) and cultured for 7 days with 10% Human AB serum, 50 ng/ml M-CSF (216-MC), and 10μM hydrocortisone (4093). Cells were harvested and stained with either (A) Mouse anti-Human VSIG4 (Catalog # MAB4646) or (B) Isotype control antibody (MAB003) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD11b Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB16991G). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> MAGH105) and cultured for 7 days with 10% Human AB serum, 50 ng/ml M-CSF (216-MC), and 10μM hydrocortisone (4093). Cells were harvested and stained with either (A) Mouse anti-Human VSIG4 (Catalog # MAB4646) or (B) Isotype control antibody (MAB003) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD11b Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB16991G). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

HAF008). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of HEK293T human embryonic kidney cell line (wild type) and HEK293T human embryonic kidney cell line transfected with human VSIG4. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46461) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

MAGH105) cultured with 50 ng/ml recombinant human M-CSF (216-MC) and 10 μM Hydrocortisone (4093) for 7 days to generate macrophages were stained with (A) Rabbit Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46461) or (B) Rabbit IgG isotype control antibody (MAB1050) followed by anti-Rabbit IgG Allophycocyanin-conjugated secondary antibody (F0111) and Mouse Anti-Human CD11b Phycoerythrin-conjugated Monoclonal Antibody (FAB16991P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> MAGH105) cultured with 50 ng/ml recombinant human M-CSF (216-MC) and 10 μM Hydrocortisone (4093) for 7 days to generate macrophages were stained with (A) Rabbit Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46461) or (B) Rabbit IgG isotype control antibody (MAB1050) followed by anti-Rabbit IgG Allophycocyanin-conjugated secondary antibody (F0111) and Mouse Anti-Human CD11b Phycoerythrin-conjugated Monoclonal Antibody (FAB16991P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

HAF018). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of HEK293T human embryonic kidney cell line (wild type) and HEK293T human embryonic kidney cell line transfected with human VSIG4. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46463) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for VSIG4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1138-SL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46462) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> 1138-SL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46462) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="Recombinant Human Siglec-7/Fc protein (1138-SL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46462) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

1138-SL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46464) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> 1138-SL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46464) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="In a functional flow cytometry test, Recombinant Human Siglec-7/Fc protein (1138-SL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46464) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />