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We offer Syntaxin 4 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each Syntaxin 4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Syntaxin 4 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the Syntaxin 4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Syntaxin 4 polyclonal antibodies and browse our Syntaxin 4 monoclonal antibody catalog.
![Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX4 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Western-Blot-NBP1-87374-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX4 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody [NBP1-87374] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87374-img0010.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0015.jpg)
![Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Western-Blot-NBP1-87374-img0007.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human lymphoid tissues shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0012.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human gastrointestinal shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0013.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0014.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0011.jpg "Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of monoclonal antibody to STX4A on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Immunocytochemistry-Immunofluorescence-H00006810-M04-img0007.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0009.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0010.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4 expression in transfected 293T cell line by STX4A monoclonal antibody (M04), clone 6D1. Lane 1: STX4 transfected lysate (Predicted MW: 34.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0012.jpg)
![Sandwich ELISA: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Detection limit for recombinant GST tagged STX4A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Sandwich-ELISA-H00006810-M04-img0008.jpg)
![Western Blot: Syntaxin 4 Antibody (6A1) [H00006810-M02] - STX4A monoclonal antibody (M02), clone 6A1. Analysis of STX4A expression in Raw 264.7.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6A1-Western-Blot-H00006810-M02-img0009.jpg "Western Blot: Syntaxin 4 Antibody (6A1) [H00006810-M02] - STX4A monoclonal antibody (M02), clone 6A1. Analysis of STX4A expression in Raw 264.7.")
![Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody (6A1) [H00006810-M02] - Analysis of monoclonal antibody to STX4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6A1-Immunocytochemistry-Immunofluorescence-H00006810-M02-img0006.jpg)
![Western Blot: Syntaxin 4 Antibody (6A1) [H00006810-M02] - STX4A monoclonal antibody (M02), clone 6A1. Analysis of STX4A expression in HeLa.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6A1-Western-Blot-H00006810-M02-img0008.jpg)
![Western Blot: Syntaxin 4 Antibody (6A1) [H00006810-M02] - STX4A monoclonal antibody (M02), clone 6A1. Analysis of STX4A expression in NIH/3T3.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6A1-Western-Blot-H00006810-M02-img0010.jpg)
![Sandwich ELISA: Syntaxin 4 Antibody (6A1) [H00006810-M02] - Detection limit for recombinant GST tagged STX4A is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6A1-Sandwich-ELISA-H00006810-M02-img0007.jpg)
![Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629] - Western blot analysis of extracts of various cell lines, using Syntaxin 4 Rabbit mAb (NBP3-16629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-8F3P7-Western-Blot-NBP3-16629-img0002.jpg "Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629] - Western blot analysis of extracts of various cell lines, using Syntaxin 4 Rabbit mAb (NBP3-16629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629] - Western blot analysis of extracts of Rat brain, using Syntaxin 4 Rabbit mAb (NBP3-16629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-8F3P7-Western-Blot-NBP3-16629-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody (8F3P7) [NBP3-16629] - Immunofluorescence analysis of NIH-3T3 cells using Syntaxin 4 Rabbit mAb (NBP3-16629) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-8F3P7-Immunocytochemistry-Immunofluorescence-NBP3-16629-img0001.jpg)
![Western Blot: Syntaxin 4 Antibody (PSH0-80) [NBP3-32990] - Western blot analysis of Syntaxin 4 on different lysates with Rabbit anti-Syntaxin 4 antibody (NBP3-32990) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: HCT 116 cell lysate (20 ug/Lane) Lane 8: U-87 MG cell lysate (20 ug/Lane) Lane 9: HUVEC cell lysate (20 ug/Lane) Lane 10: NIH/3T3 cell lysate (20 ug/Lane) Lane 11: RAW264.7 cell lysate (20 ug/Lane) Lane 12: PC-12 cell lysate (20 ug/Lane) Lane 13: Mouse kidney tissue lysate (40 ug/Lane) Lane 14: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34/65 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32990) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32990_rabbit-syntaxin-4-mab-psh0-80-288202412493446.jpg "Western Blot: Syntaxin 4 Antibody (PSH0-80) [NBP3-32990] - Western blot analysis of Syntaxin 4 on different lysates with Rabbit anti-Syntaxin 4 antibody (NBP3-32990) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: HCT 116 cell lysate (20 ug/Lane) Lane 8: U-87 MG cell lysate (20 ug/Lane) Lane 9: HUVEC cell lysate (20 ug/Lane) Lane 10: NIH/3T3 cell lysate (20 ug/Lane) Lane 11: RAW264.7 cell lysate (20 ug/Lane) Lane 12: PC-12 cell lysate (20 ug/Lane) Lane 13: Mouse kidney tissue lysate (40 ug/Lane) Lane 14: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34/65 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32990) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Syntaxin 4 Antibody (PSH0-80) [NBP3-32990] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Syntaxin 4 antibody (NBP3-32990) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32990) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32990_rabbit-syntaxin-4-mab-psh0-80-298202414304178.jpg)
![Immunohistochemistry: Syntaxin 4 Antibody (PSH0-80) [NBP3-32990] - Immunofluorescence analysis of paraffin-embedded mouse small intestine tissue labeling Syntaxin 4 with Rabbit anti-Syntaxin 4 antibody (NBP3-32990) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32990, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32990_rabbit-syntaxin-4-mab-psh0-80-298202414262621.jpg)
![Western Blot: Syntaxin 4 Antibody (JE35-92) [NBP3-32989] - Western blot analysis of Syntaxin 4 on different lysates with Rabbit anti-Syntaxin 4 antibody (NBP3-32989) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: U-87 MG cell lysate (20 ug/Lane) Lane 7: Human lung tissue lysate (40 ug/Lane) Lane 8: Mouse skin tissue lysate (40 ug/Lane) Lane 9: rat brain tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34/30 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32989) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32989_rabbit-syntaxin-4-mab-je35-92-288202412493415.jpg "Western Blot: Syntaxin 4 Antibody (JE35-92) [NBP3-32989] - Western blot analysis of Syntaxin 4 on different lysates with Rabbit anti-Syntaxin 4 antibody (NBP3-32989) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: U-87 MG cell lysate (20 ug/Lane) Lane 7: Human lung tissue lysate (40 ug/Lane) Lane 8: Mouse skin tissue lysate (40 ug/Lane) Lane 9: rat brain tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34/30 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32989) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Syntaxin 4 Antibody (JE35-92) [NBP3-32989] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Syntaxin 4 antibody (NBP3-32989) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32989) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32989_rabbit-syntaxin-4-mab-je35-92-298202414262661.jpg)
![Immunoprecipitation: Syntaxin 4 Antibody [NBP2-32227] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Syntaxin 4 antibody NBP2-32227 used for IP at 6 ug per reaction. For blotting immunoprecipitated Syntaxin 4 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunoprecipitation-NBP2-32227-img0001.jpg "Immunoprecipitation: Syntaxin 4 Antibody [NBP2-32227] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Syntaxin 4 antibody NBP2-32227 used for IP at 6 ug per reaction. For blotting immunoprecipitated Syntaxin 4 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Syntaxin 4 Antibody (S07-1G2) [NBP3-15096] - Western blot detection of Syntaxin 4 in C6, 3T3, Hela cell lysates using Syntaxin 4 Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-S07-1G2-Western-Blot-NBP3-15096-img0001.jpg "Western Blot: Syntaxin 4 Antibody (S07-1G2) [NBP3-15096] - Western blot detection of Syntaxin 4 in C6, 3T3, Hela cell lysates using Syntaxin 4 Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.")
![Western Blot: Syntaxin 4 Antibody [NBP1-69179] - Human Muscle lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Western-Blot-NBP1-69179-img0002.jpg "Western Blot: Syntaxin 4 Antibody [NBP1-69179] - Human Muscle lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Syntaxin 4 Antibody [NBP1-69149] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Western-Blot-NBP1-69149-img0003.jpg "Western Blot: Syntaxin 4 Antibody [NBP1-69149] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")