HAF016). A specific band was detected for a-2,8-Sialyltransferase 8B/ST8SIA2 at approximately 63 to 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for a-2,8-Sialyltransferase 8B/ST8SIA2 at approximately 63 to 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, Saos-2 human osteosarcoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-2,8-Sialyltransferase 8B/ST8SIA2 at approximately 63 to 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />