SHMT2 Antibodies
We offer SHMT2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each SHMT2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SHMT2 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the SHMT2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SHMT2 polyclonal antibodies and browse our SHMT2 monoclonal antibody catalog.
Alternate Names for SHMT2 Antibodies
anti-SHMT2 antibody, anti-EC 2.1.2.1 antibody, anti-GLY A+ antibody, anti-GLYA antibody, anti-glycine auxotroph A, human complement for hamster antibody, anti-Glycine hydroxymethyltransferase antibody, anti-serine aldolase antibody, anti-serine hydroxymethylase antibody, anti-serine hydroxymethyltransferase 2 (mitochondrial) antibody, anti-serine hydroxymethyltransferase, mitochondrial antibody, anti-Serine methylase antibody, anti-SHMT antibody, anti-threonine aldolase antibody
12 Results for "shmt2" in Primary Antibodies
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80755] - Analysis in human liver and skeletal muscle tissues using NBP1-80755 antibody. Corresponding SHMT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80755-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80755] - Analysis in human liver and skeletal muscle tissues using NBP1-80755 antibody. Corresponding SHMT2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80755] - Staining of human duodenum, liver, lymph node and skeletal muscle using Anti-SHMT2 antibody NBP1-80755 (A) shows similar protein distribution across tissues to independent antibody NBP1-80754 (B).](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80755-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SHMT2 Antibody [NBP1-80755] - Analysis using Anti-SHMT2 antibody NBP1-80755 (A) shows similar pattern to independent antibody NBP1-80754 (B).](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-NBP1-80755-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SHMT2 Antibody [NBP1-80755] - Staining of human cell line U-2 OS shows localization to mitochondria & microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80755-img0012.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80755] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80755-img0024.jpg)
![Western Blot: SHMT2 Antibody [NBP1-80755] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-NBP1-80755-img0009.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80755] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80755-img0021.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80755] - Staining of human lymph node shows moderate granular cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80755-img0022.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80755] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80755-img0023.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining in human liver and skeletal muscle tissues . Corresponding SHMT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining in human liver and skeletal muscle tissues . Corresponding SHMT2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human duodenum, liver, lymph node and skeletal muscle using Anti-SHMT2 antibody NBP1-80754 (A) shows similar protein distribution across tissues to independent antibody NBP1-80755 (B).](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0027.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SHMT2 Antibody [NBP1-80754] - Analysis using Anti-SHMT2 antibody NBP1-80754 (A) shows similar pattern to independent antibody NBP1-80755 (B).](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-NBP1-80754-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: SHMT2 Antibody [NBP1-80754] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80754-img0009.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0028.jpg)
![Western Blot: SHMT2 Antibody [NBP1-80754] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-NBP1-80754-img0007.jpg)
![Western Blot: SHMT2 Antibody [NBP1-80754] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-NBP1-80754-img0010.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0016.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human lymph node shows moderate granular cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0017.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0018.jpg)
![Immunohistochemistry: SHMT2 Antibody [NBP3-37977] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using SHMT2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37977_rabbit-shmt2-pab-10122024120649.jpg "Immunohistochemistry: SHMT2 Antibody [NBP3-37977] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using SHMT2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: SHMT2 Antibody [NBP3-37977] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using SHMT2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37977_rabbit-shmt2-pab-10122024120668.jpg)
![Western Blot: SHMT2 Antibody [NBP3-37977] - Western blot analysis of lysates from SW620 cells, using SHMT2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-37977_rabbit-shmt2-pab-101220241243942.jpg)
![Immunocytochemistry/ Immunofluorescence: SHMT2 Antibody [NBP3-37977] - Immunofluorescence analysis of L929 cells using SHMT2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37977_rabbit-shmt2-pab-1012202411545573.jpg)
![Immunoprecipitation: SHMT2 Antibody [NBP3-37977] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug SHMT2 antibody. Western blot was performed from the immunoprecipitate using SHMT2 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-37977_rabbit-shmt2-pab-101220241252887.jpg)
![Immunohistochemistry: SHMT2 Antibody [NBP3-37977] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using SHMT2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37977_rabbit-shmt2-pab-1012202411535824.jpg)
![Immunocytochemistry/ Immunofluorescence: SHMT2 Antibody [NBP3-37977] - Immunofluorescence analysis of U-2 OS cells using SHMT2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37977_rabbit-shmt2-pab-1012202411535814.jpg)
![Western Blot: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHMT2.](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Western-Blot-NBP2-45826-img0013.jpg "Western Blot: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHMT2.")
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0012.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0001.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0002.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0003.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Adenocarcinoma of Human ovary tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0004.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0005.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0006.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0007.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0008.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0009.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0010.jpg)
![Immunohistochemistry: SHMT2 Antibody (3E9) [NBP2-45826] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/SHMT2-Antibody-3E9-Immunohistochemistry-NBP2-45826-img0011.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP2-20354] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP2-20354-img0002.jpg "Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP2-20354] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.")
![Western Blot: SHMT2 Antibody [NBP2-20354] - Sample (whole cell lysate) A: 293T 10ug, B: HeLa 10ug, 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-NBP2-20354-img0001.jpg)
![Western Blot: SHMT2 Antibody [H00006472-B01P] - Analysis of SHMT2 expression in human spleen.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-H00006472-B01P-img0003.jpg "Western Blot: SHMT2 Antibody [H00006472-B01P] - Analysis of SHMT2 expression in human spleen.")
![Immunocytochemistry/Immunofluorescence: SHMT2 Antibody [H00006472-B01P] - Analysis of purified antibody to SHMT2 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunocytochemistry-Immunofluorescence-H00006472-B01P-img0001.jpg)
![Western Blot: SHMT2 Antibody [H00006472-B01P] - Analysis of SHMT2 expression in HeLa.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-H00006472-B01P-img0002.jpg)
![Western Blot: SHMT2 Antibody [H00006472-B01P] - Analysis of SHMT2 expression in transfected 293T cell line by SHMT2 polyclonal antibody. Lane 1: SHMT2 transfected lysate(55.44 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-H00006472-B01P-img0004.jpg)
![Western Blot: SHMT2 Antibody [NBP3-35223] - Western blot analysis of various lysates using SHMT2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35223_rabbit-shmt2-pab-912202414123876.jpg "Western Blot: SHMT2 Antibody [NBP3-35223] - Western blot analysis of various lysates using SHMT2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.")
![<b>Genetic Strategies Validation. </b>Western Blot: SHMT2 Antibody [NBP3-35223] - Western Blot analysis of lysates from wild type (WT) and SHMT2 knockout (KO) HeLa cells using [KO Validated] SHMT2 Rabbit pAb at 1:400 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-35223_rabbit-shmt2-pab-31120251704769.jpg)
![Western Blot: SHMT2 Antibody [NBP3-35223] - Western Blot analysis of various lysates using [KO Validated] SHMT2 Rabbit pAb at 1:400 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-35223_rabbit-shmt2-pab-31120251744425.jpg)
![Western Blot: SHMT2 Antibody (5E7) [H00006472-M04-100ug] - Detection against Immunogen (37.07 KDa) .](http://images.novusbio.com/fullsize/SHMT2-Antibody-5E7-Western-Blot-H00006472-M04-100ug-img0003.jpg "Western Blot: SHMT2 Antibody (5E7) [H00006472-M04-100ug] - Detection against Immunogen (37.07 KDa) .")
![Western Blot: SHMT2 Antibody (5E7) [H00006472-M04-100ug] - SHMT2 monoclonal antibody (M04), clone 5E7. Western Blot analysis of SHMT2 expression in HeLa.](http://images.novusbio.com/fullsize/SHMT2-Antibody-5E7-Western-Blot-H00006472-M04-100ug-img0002.jpg)
![ELISA: SHMT2 Antibody (5E7) [H00006472-M04-100ug] - Detection limit for recombinant GST tagged SHMT2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SHMT2-Antibody-5E7-ELISA-H00006472-M04-100ug-img0001.jpg)
![Immunoprecipitation:SHMT2 AntibodyNBP3-30303] -Detection of human SHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHMT2 antibody NBP3-30303 (lot NBP3-30303-1) used for IP at 6 µg per reaction. SHMT2 was also immunoprecipitated by rabbit anti-SHMT2.. For blotting immunoprecipitated SHMT2, NBP3-30303 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30303_rabbit-shmt2-pab-3012202418284610.jpg "Immunoprecipitation:SHMT2 AntibodyNBP3-30303] -Detection of human SHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHMT2 antibody NBP3-30303 (lot NBP3-30303-1) used for IP at 6 µg per reaction. SHMT2 was also immunoprecipitated by rabbit anti-SHMT2.. For blotting immunoprecipitated SHMT2, NBP3-30303 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:SHMT2 AntibodyNBP3-30380] -Detection of human SHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHMT2 antibody NBP3-30380 (lot NBP3-30380-1) used for IP at 6 µg per reaction. SHMT2 was also immunoprecipitated by rabbit anti-SHMT2.. For blotting immunoprecipitated SHMT2, NBP3-30380 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30380_rabbit-shmt2-pab-301220241836172.jpg "Immunoprecipitation:SHMT2 AntibodyNBP3-30380] -Detection of human SHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHMT2 antibody NBP3-30380 (lot NBP3-30380-1) used for IP at 6 µg per reaction. SHMT2 was also immunoprecipitated by rabbit anti-SHMT2.. For blotting immunoprecipitated SHMT2, NBP3-30380 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")