SAV1 Antibodies
We offer SAV1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each SAV1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SAV1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the SAV1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SAV1 polyclonal antibodies and browse our SAV1 monoclonal antibody catalog.
Alternate Names for SAV1 Antibodies
anti-SAV1 antibody, anti-45 kDa WW domain protein antibody, anti-hWW45 antibody, anti-protein salvador homolog 1 antibody, anti-salvador homolog 1 (Drosophila) antibody, anti-SAV antibody, anti-WW domain-containing antibody, anti-WW45salvador antibody, anti-WWP4,1700040G09Rik antibody
7 Results for "sav1" in Primary Antibodies
![Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - MST2 and SAV1 increase the levels of PPAR-gamma by increasing its protein stability. Increasing amounts (0.5, 1, 3 ug) of Myc-SAV1 were co-expressed with Flag-PPAR gamma and/or HA-MST2 in 293 cells. At 48 h after transfection, cell lysates were immunoblotted with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0030983), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Western-Blot-H00060485-M02-img0012.jpg "Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - MST2 and SAV1 increase the levels of PPAR-gamma by increasing its protein stability. Increasing amounts (0.5, 1, 3 ug) of Myc-SAV1 were co-expressed with Flag-PPAR gamma and/or HA-MST2 in 293 cells. At 48 h after transfection, cell lysates were immunoblotted with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0030983), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: SAV1 Antibody (3B2) [H00060485-M02] - Analysis of monoclonal antibody to SAV1 on HeLa cell. Antibody concentration 60 ug/ml.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Immunocytochemistry-Immunofluorescence-H00060485-M02-img0006.jpg)
![Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - SAV1 monoclonal antibody (M02), clone 3B2. Analysis of SAV1 expression in HepG2.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Western-Blot-H00060485-M02-img0009.jpg)
![Immunoprecipitation: SAV1 Antibody (3B2) [H00060485-M02] - Analysis of SAV1 transfected lysate using anti-SAV1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SAV1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Immunoprecipitation-H00060485-M02-img0007.jpg)
![ELISA: SAV1 Antibody (3B2) [H00060485-M02] - Detection limit for recombinant GST tagged SAV1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-ELISA-H00060485-M02-img0011.jpg)
![Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - MST2 & SAV1 increase the levels of PPAR gamma by increasing its protein stability.(A) Increasing amounts (0.5, 1, 3 µg) of Myc-SAV1 were co-expressed with Flag-PPAR gamma and/or HA-MST2 in 293 cells. At 48 h after transfection, cell lysates were immunoblotted with the indicated antibodies. (B) Flag-tagged PPAR gamma or PPAR gamma (128–505) was co-expressed with HA-MST2, HA-MST2-KD (inactive mutant), Myc-SAV1, and/or Myc-SAV1(1–201) in 293 cells as indicated. Cell lysates were prepared at various times after treatment with 40 µg/mL cycloheximide & then immunoblotted with an anti-Flag antibody. The PPAR gamma protein expression was quantified & the half-life of PPAR gamma protein was calculated. Expression of GAPDH was detected as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22292086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00060485-m02_mouse-monoclonal-sav1-antibody-3b2-310202415371988.jpg)
![Western Blot: SAV1 Antibody - Azide and BSA Free [SAV1] - Western blot analysis of lysates from wild type (WT) and SAV1 knockout (KO) HeLa(KO) cells, using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 50s.](http://images.novusbio.com/fullsize/nbp3-15584_rabbit-sav1-pab-5220251734423.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SAV1 Antibody - Azide and BSA Free [SAV1] - Western blot analysis of lysates from wild type (WT) and SAV1 knockout (KO) HeLa(KO) cells, using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 50s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Human colon using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunohistochemistry-Paraffin-NBP3-15584-img0004.jpg)
![Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Mouse kidney using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunohistochemistry-Paraffin-NBP3-15584-img0002.jpg)
![Immunohistochemistry-Paraffin: SAV1 Antibody [NBP3-15584] - Immunohistochemistry of paraffin-embedded Rat kidney using [KO Validated] SAV1 Rabbit pAb (NBP3-15584) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunohistochemistry-Paraffin-NBP3-15584-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SAV1 Antibody [NBP3-15584] - Western blot analysis of extracts from normal (control) and [KO Validated] SAV1 knockout (KO) HeLa cells, using [KO Validated] SAV1 antibody (NBP3-15584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/SAV1-Antibody-Knockout-Validated-NBP3-15584-img0001.jpg)
![Western Blot: SAV1 Antibody [NBP2-13282] - Analysis in human cell lines U2OS and A-549 using anti-SAV1 antibody. Corresponding SAV1 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.](http://images.novusbio.com/fullsize/SAV1-Antibody-Western-Blot-NBP2-13282-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SAV1 Antibody [NBP2-13282] - Analysis in human cell lines U2OS and A-549 using anti-SAV1 antibody. Corresponding SAV1 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SAV1 Antibody [NBP2-13282] - Staining of human cell line SiHa shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13282-img0006.jpg)
![Immunohistochemistry-Paraffin: SAV1 Antibody [NBP2-13282] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SAV1-Antibody-Immunohistochemistry-Paraffin-NBP2-13282-img0008.jpg)
![Western Blot: SAV1 Antibody [NBP2-13282] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/SAV1-Antibody-Western-Blot-NBP2-13282-img0010.jpg)
![Immunohistochemistry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32931) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-298202414304199.jpg "Immunohistochemistry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32931) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: SAV1 Antibody (PSH04-45) [NBP3-32931] - Flow cytometric analysis of HCT 116 cells labeling SAV1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32931, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-392024884625.jpg)
![Western Blot: SAV1 Antibody (PSH04-45) [NBP3-32931] - Western blot analysis of SAV1 on different lysates with Rabbit anti-SAV1 antibody (NBP3-32931) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: MDA-MB-231 cell lysate (20 µg/Lane) Lane 6: SW480 cell lysate (20 µg/Lane) Lane 7: BxPC-3 cell lysate (20 µg/Lane) Lane 8: 786-0 cell lysate (negative) (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: NIH/3T3 cell lysate (20 µg/Lane) Lane 11: PC-12 cell lysate (20 µg/Lane) Lane 12: Mouse testis tissue lysate (40 µg/Lane) Lane 13: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minute; ECL: 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32931_rabbit-sav1-mab-psh04-45-3920248453522.jpg)
![Western Blot: SAV1 Antibody (2B7) [NBP2-45687] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAV1 .](http://images.novusbio.com/fullsize/SAV1-Antibody-2B7-Western-Blot-NBP2-45687-img0002.jpg "Western Blot: SAV1 Antibody (2B7) [NBP2-45687] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAV1 .")
![Immunohistochemistry: SAV1 Antibody (2B7) [NBP2-45687] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0) Dilution: 1:150](http://images.novusbio.com/fullsize/SAV1-Antibody-2B7-Immunohistochemistry-NBP2-45687-img0001.jpg)
![Western Blot: SAV1 Antibody (3A10) [H00060485-M04] - Analysis of SAV1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SAV1-Antibody-3A10-Western-Blot-H00060485-M04-img0003.jpg "Western Blot: SAV1 Antibody (3A10) [H00060485-M04] - Analysis of SAV1 expression in Hela S3 NE (Cat # L013V3).")
![Sandwich ELISA: SAV1 Antibody (3A10) [H00060485-M04] - Detection limit for recombinant GST tagged SAV1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SAV1-Antibody-3A10-Sandwich-ELISA-H00060485-M04-img0002.jpg)
![Western Blot: SAV1 Antibody [NBP3-38635] - Western blot analysis of various lysates using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38635_rabbit-sav1-pab-912202415161853.jpg "Western Blot: SAV1 Antibody [NBP3-38635] - Western blot analysis of various lysates using SAV1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")