Rad17 Antibodies
We offer Rad17 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each Rad17 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Rad17 Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rat, Yeast. Use the list below to choose the Rad17 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Rad17 polyclonal antibodies and browse our Rad17 monoclonal antibody catalog.
Alternate Names for Rad17 Antibodies
anti-Rad17 antibody, anti-CCYC antibody, anti-cell cycle checkpoint protein (RAD17) antibody, anti-cell cycle checkpoint protein RAD17 antibody, anti-FLJ41520 antibody, anti-HRAD17 antibody, anti-R24L antibody, anti-RAD1 (S. pombe) homolog antibody, anti-RAD1 homolog antibody, anti-RAD17 homolog (S. pombe) antibody, anti-Rad17-like protein antibody, anti-RAD17SP antibody, anti-RAD24 antibody, anti-RF-C activator 1 homolog antibody, anti-RF-C/activator 1 homolog antibody
31 Results for "rad17" in Primary Antibodies
AF1926). For additional reference, Western blot (right panel) shows lysates of K562 cell line probed with 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). View our recommended buffer recipes for immunoprecipitation." alt="Rad17 was immunoprecipitated from 500 µg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2-3 µg Mouse Anti-Human Rad17 Monoclonal Antibody (Catalog # MAB1926) for 1 hour on ice. Rad17-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Rad17 (left panel) was detected by Western blot using 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). For additional reference, Western blot (right panel) shows lysates of K562 cell line probed with 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). View our recommended buffer recipes for immunoprecipitation." class="big_thumb" />
![Western Blot: Rad17 [p Ser645] Antibody [NBP2-24457] - analysis of phosphorylated Rad17 (Ser645) in 1) untreated PANC-1 cells (a human pancreatic carcinoma) and 2) UV-treated PANC-1 cells using this antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NBP2-24457-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NBP2-24457] - analysis of phosphorylated Rad17 (Ser645) in 1) untreated PANC-1 cells (a human pancreatic carcinoma) and 2) UV-treated PANC-1 cells using this antibody at 0.5 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NBP2-24457] - Western blot analysis of phosphorylated Rad17 (Ser645) in recombinant fusion protein containing 1) a phosphorylated serine at position 645 and 2) an unphosphorylated serine at position 645 using NBP2-24457 at 0.025 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NBP2-24457-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad17 [p Ser645] Antibody [NBP2-24457] - Analysis of phosphorylated Rad17 in formalin-fixed, paraffin-embedded mouse liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-Paraffin-NBP2-24457-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rad17 Antibody [NBP1-86561] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Rad17-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86561-img0006.jpg "Immunocytochemistry/Immunofluorescence: Rad17 Antibody [NBP1-86561] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Rad17 Antibody [NBP1-86561] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/Rad17-Antibody-Immunohistochemistry-Paraffin-NBP1-86561-img0001.jpg)
![Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0045.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0049.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Whole cell lysate (50 ug) from HeLa cells before (-) or after (+) treatment with 10 Gy ionizing radiation. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0002.jpg)
![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0048.jpg)
![Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.](http://images.novusbio.com/fullsize/Rad17-Antibody-Western-Blot-NBP2-27106-img0002.jpg "Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.")
![Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Western-Blot-NBP3-13072-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunohistochemistry-Paraffin-NBP3-13072-img0003.jpg)
![Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunohistochemistry-Paraffin-NBP3-13072-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Immunocytochemistry analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Rad17 with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32792_rabbit-rad17-p-ser656-mab-je63-30-2920241114833.jpg "Immunocytochemistry/ Immunofluorescence: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Immunocytochemistry analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Rad17 with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.")
![Flow Cytometry: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Flow cytometric analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Rad17.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32792, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32792_rabbit-rad17-p-ser656-mab-je63-30-29202416453256.jpg)
![Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500](http://images.novusbio.com/fullsize/Rad17-Antibody-Western-Blot-NB100-173-img0001.jpg "Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500")
![Western Blot: Rad17 [p Ser 656] Antibody (SR2259) [NBP3-21602] - Western blot analysis using NBP3-21602 in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21602_rabbit-rad17-p-ser-656-mab-sr2259-67202310620.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser 656] Antibody (SR2259) [NBP3-21602] - Western blot analysis using NBP3-21602 in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate.")
