PPP3CB Antibodies
We offer PPP3CB Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each PPP3CB Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PPP3CB Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish. Use the list below to choose the PPP3CB Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PPP3CB polyclonal antibodies and browse our PPP3CB monoclonal antibody catalog.
Alternate Names for PPP3CB Antibodies
anti-PPP3CB antibody, anti-Calmodulin-dependent calcineurin A subunit beta isoform antibody, anti-CALNA2protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform(calcineurin A beta) antibody, anti-CAM-PRP catalytic subunit antibody, anti-CNA2catalytic subunit, beta isoform antibody, anti-EC 3.1.3.16 antibody, anti-protein phosphatase 2B, catalytic subunit, beta isoform antibody, anti-protein phosphatase 3, catalytic subunit, beta isozyme antibody, anti-protein phosphatase from PCR fragment H32 antibody, anti-serine/threonine-protein phosphatase 2B catalytic subunit beta isoform antibody
7 Results for "ppp3cb" in Primary Antibodies
![Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-288202412442938.png "Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-PPP3CB antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29820241426262.png)
![Immunocytochemistry/ Immunofluorescence: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunocytochemistry analysis of MCF-7 cells labeling PPP3CB with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-2920241114810.png)
![Flow Cytometry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Flow cytometric analysis of MCF-7 cells labeling PPP3CB. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32848, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32848_rabbit-ppp3cb-mab-psh0-21-29202416372124.png)
![Western Blot: PPP3CB Antibody (5D3) [H00005532-M01] - Analysis of PPP3CB expression in transfected 293T cell line by PPP3CB monoclonal antibody (M01), clone 5D3.Lane 1: PPP3CB transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PPP3CB-Antibody-5D3-Western-Blot-H00005532-M01-img0006.jpg "Western Blot: PPP3CB Antibody (5D3) [H00005532-M01] - Analysis of PPP3CB expression in transfected 293T cell line by PPP3CB monoclonal antibody (M01), clone 5D3.Lane 1: PPP3CB transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: PPP3CB Antibody (5D3) [H00005532-M01] - Analysis of PPP3CB over-expressed 293 cell line, cotransfected with PPP3CB Validated Chimera RNAi ( Cat # H00005532-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPP3CB monoclonal antibody (M01), clone 5D3 (Cat # H00005532-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PPP3CB-Antibody-5D3-Western-Blot-H00005532-M01-img0004.jpg)
![Sandwich ELISA: PPP3CB Antibody (5D3) [H00005532-M01] - Detection limit for recombinant GST tagged PPP3CB is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PPP3CB-Antibody-5D3-Sandwich-ELISA-H00005532-M01-img0005.jpg)
![Western Blot: PPP3CB Antibody [NBP2-58920] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/PPP3CB-Antibody-Western-Blot-NBP2-58920-img0002.jpg "Western Blot: PPP3CB Antibody [NBP2-58920] - Western blot analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: PPP3CB Antibody [NBP2-58920] - Staining of human cell line U-251 MG shows localization to mitochondria.](http://images.novusbio.com/fullsize/PPP3CB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58920-img0001.jpg)
![Immunohistochemistry: PPP3CB Antibody [NBP1-86657] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PPP3CB-Antibody-Immunohistochemistry-NBP1-86657-img0003.jpg "Immunohistochemistry: PPP3CB Antibody [NBP1-86657] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.")
![Western Blot: PPP3CB Antibody [NBP1-74253] - Mouse Heart Lysate 1ug/ml Gel Concentration 12%](http://images.novusbio.com/fullsize/PPP3CB-Antibody-Western-Blot-NBP1-74253-img0001.jpg "Western Blot: PPP3CB Antibody [NBP1-74253] - Mouse Heart Lysate 1ug/ml Gel Concentration 12%")