PILR-alpha Antibodies

HAF008). A specific band was detected for PILR-a at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for PILR-a at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs), human spleen tissue, human lung tissue, CEM human T-lymphoblastoid cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human PILR-a Monoclonal Antibody (Catalog # MAB64841) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PILR-a at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox"> AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse PILR-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4318, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

6484-PR) binds to immobilized recombinant human PANP Fc Chimera coated at 5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PILR-a Monoclonal Antibody (Catalog # MAB64842). At 1-5 µg/mL, this antibody will block 50% of the binding." class="big_lightbox"> 6484-PR) binds to immobilized recombinant human PANP Fc Chimera coated at 5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PILR-a Monoclonal Antibody (Catalog # MAB64842). At 1-5 µg/mL, this antibody will block 50% of the binding." alt="In a functional ELISA, Recombinant Human PILR-a (Catalog # 6484-PR) binds to immobilized recombinant human PANP Fc Chimera coated at 5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PILR-a Monoclonal Antibody (Catalog # MAB64842). At 1-5 µg/mL, this antibody will block 50% of the binding." class="big_thumb" />

F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050)." class="big_lightbox"> F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050)." class="big_thumb" />

5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." class="big_lightbox"> 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." alt="Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6484, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." class="big_thumb" />

HAF007). A specific band was detected for PILR-a at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for PILR-a at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human PILR-a Monoclonal Antibody (Catalog # MAB6484) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PILR-a at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />