NF-L Antibodies
We offer NF-L Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Simple Western, Western Blot. Each NF-L Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NF-L Antibodies can be used in a variety of model species: Avian, Bovine, Chicken, Feline, Human, Mammal, Mouse, Porcine, Primate, Rat. Use the list below to choose the NF-L Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NF-L polyclonal antibodies and browse our NF-L monoclonal antibody catalog.
Alternate Names for NF-L Antibodies
anti-NF-L antibody, anti-NEFL antibody, anti-68 kDa neurofilament protein antibody, anti-neurofilament protein, light chain antibody, anti-neurofilament subunit NF-L antibody, anti-Neurofilament triplet L protein antibody, anti-neurofilament, light polypeptide antibody, anti-neurofilament-light antibody, anti-NF68FLJ53642 antibody, anti-NFLlight polypeptide 68kDa antibody
27 Results for "nf-l" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) [NBP2-44891] - Immunofluorescence staining of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunocytochemistry-Immunofluorescence-NBP2-44891-img0005.jpg "Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) [NBP2-44891] - Immunofluorescence staining of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).")
![Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) [NBP2-44891] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunohistochemistry-Paraffin-NBP2-44891-img0002.jpg)
![Flow Cytometry: NF-L Antibody (NR-4) [NBP2-44891] - Flow Cytometric Analysis of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Flow-Cytometry-NBP2-44891-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) [NBP2-44891] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunohistochemistry-Paraffin-NBP2-44891-img0001.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-54669 antibody. Corresponding NEFL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494437..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-54669 antibody. Corresponding NEFL RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NF-L Antibody [NBP2-54669] - Analysis in control (vector only transfected HEK293T lysate) and NEFL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NBP2-54669-img0006.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human cerebral cortex shows strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494530..jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human cerebellum shows strong positivity in neuronal processes in molecular layer.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494628..jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494721..jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-54669_rabbit-polyclonal-nf-l-antibody-immunohistochemistry-paraffin-52202494818..jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47970-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - HEK293 cells using Neurofilament Monoclonal Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47970-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) [NBP2-44893] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Immunohistochemistry-Paraffin-NBP2-44893-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) [NBP2-44893] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) [NBP2-44893] - Flow Cytometric Analysis of HEK293 cells using NF-L Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Flow-Cytometry-NBP2-44893-img0002.jpg)
![Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-278202415212420.jpg "Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-29820241420994.jpg)
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32650, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-298202414262675.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PS02-10) [NBP3-32650] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-2920241192341.jpg)
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) [NBP2-44892] - Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).](http://images.novusbio.com/fullsize/NF-L-Antibody-SPM204-Immunohistochemistry-Paraffin-NBP2-44892-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) [NBP2-44892] - Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).")
![Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) - IHC-Prediluted [NBP2-48293] - Human cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).](http://images.novusbio.com/fullsize/NF-L-Antibody-SPM204-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48293-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) - IHC-Prediluted [NBP2-48293] - Human cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).")
![Immunohistochemistry-Paraffin: NF-L Antibody (NEFL/2983R) [NBP3-07407] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-L Rabbit Monoclonal Antibody (NEFL/2983R).](http://images.novusbio.com/fullsize/NF-L-Antibody-NEFL-2983R-Immunohistochemistry-Paraffin-NBP3-07407-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NEFL/2983R) [NBP3-07407] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-L Rabbit Monoclonal Antibody (NEFL/2983R).")