MDR1/ABCB1 Antibodies
We offer MDR1/ABCB1 Antibodies for use in common research applications: Blocking/Neutralizing, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Sandwich ELISA, Western Blot. Each MDR1/ABCB1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MDR1/ABCB1 Antibodies can be used in a variety of model species: Canine, Hamster, Human, Monkey, Mouse, Primate, Rat. Use the list below to choose the MDR1/ABCB1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MDR1/ABCB1 polyclonal antibodies and browse our MDR1/ABCB1 monoclonal antibody catalog.
Alternate Names for MDR1/ABCB1 Antibodies
anti-MDR1/ABCB1 antibody, anti-ABCB1 antibody, anti-ABC20 antibody, anti-ATP-binding cassette sub-family B member 1 antibody, anti-ATP-binding cassette, sub-family B (MDR/TAP), member 1 antibody, anti-CD243 antibody, anti-CD243 antigen antibody, anti-CLCS antibody, anti-colchicin sensitivity antibody, anti-doxorubicin resistance antibody, anti-EC 3.6.3 antibody, anti-EC 3.6.3.44 antibody, anti-GP170 antibody, anti-IBD13 antibody, anti-MDR1MGC163296 antibody, anti-multidrug resistance protein 1 antibody, anti-P-glycoprotein 1 antibody, anti-P-gp antibody, anti-PGY1P-GP antibody
23 Results for "mdr1-abcb1" in Primary Antibodies
![Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Western-Blot-NBP2-67667-img0008.jpg "Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0007.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0001.jpg)
![Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight incubation. Secondary antibody at 2 ug/mL for 1.5 hour (Invitrogen A-11012). Mounting with Vector Vectashield with DAPI. Image from a verified customer review.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Frozen-NBP2-67667-img0004.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0003.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0005.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Monoclonal MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Human brain microvascular endothelial cells stained for ABCB1, red and ciliary MDR1/ABCB1 green and nuclear DAPI blue. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67667_rabbit-monoclonal-mdr1-abcb1-antibody-sn06-42-immunocytochemistry-immunofluorescence-22202415364..png)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding MDR1/ABCB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding MDR1/ABCB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90291-img0004.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0014.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0010.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human kidney shows moderate positivity in apical membrane in cells in distal tubules.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0011.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human liver shows weak membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0012.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0013.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NB100-80870-img0003.jpg "Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.")
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0015.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0001.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0002.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0003.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0004.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0005.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0006.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human lung tissue within the normal limits using ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0007.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0008.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0009.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0010.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0011.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0012.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0013.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0014.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0016.jpg)
![Western Blot: MDR1/ABCB1 Antibody (3B2) [NBP2-46465] - SF9 cells lysate (5 ug, left lane) and SF9 cells lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 995-1280 of human ABCB1 (NP_000918).](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-3B2-Western-Blot-NBP2-46465-img0003.jpg "Western Blot: MDR1/ABCB1 Antibody (3B2) [NBP2-46465] - SF9 cells lysate (5 ug, left lane) and SF9 cells lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 995-1280 of human ABCB1 (NP_000918).")
![Immunohistochemistry: MDR1/ABCB1 Antibody (3B2) [NBP2-46465] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-3B2-Immunohistochemistry-NBP2-46465-img0002.jpg)
![Immunohistochemistry: MDR1/ABCB1 Antibody (3B2) [NBP2-46465] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-3B2-Immunohistochemistry-NBP2-46465-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: P Glycoprotein protein stained by P Glycoprotein antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17251-img0003.jpg "Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: P Glycoprotein protein stained by P Glycoprotein antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Western Blot: MDR1/ABCB1 Antibody [NBP2-17251] - Unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MDR1/ABCB1 antibody (NBP2-17251) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp2-17251_rabbit-polyclonal-mdr1-abcb1-antibody-910202419593571.jpg)
![Western Blot: MDR1/ABCB1 Antibody (6G11C12) [NBP2-37294] - Analysis using ABCB1 mAb against HEK293 (1) and ABCB1 (AA: 632-693)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11C12-Western-Blot-NBP2-37294-img0003.jpg "Western Blot: MDR1/ABCB1 Antibody (6G11C12) [NBP2-37294] - Analysis using ABCB1 mAb against HEK293 (1) and ABCB1 (AA: 632-693)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: MDR1/ABCB1 Antibody (6G11C12) [NBP2-37294] - Analysis using ABCB1 mAb against human ABCB1 (AA: 632-693) recombinant protein. (Expected MW is 32.4 kDa).](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11C12-Western-Blot-NBP2-37294-img0002.jpg)
![ELISA: MDR1/ABCB1 Antibody (6G11C12) [NBP2-37294] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11C12-ELISA-NBP2-37294-img0001.jpg)
![Western Blot: MDR1/ABCB1 Antibody (6G11F8) [NBP2-37290] - Western blot analysis using ABCB1 mAb against human ABCB1 (AA: 632-693) recombinant protein. (Expected MW is 32.4 kDa)](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11F8-Western-Blot-NBP2-37290-img0002.jpg "Western Blot: MDR1/ABCB1 Antibody (6G11F8) [NBP2-37290] - Western blot analysis using ABCB1 mAb against human ABCB1 (AA: 632-693) recombinant protein. (Expected MW is 32.4 kDa)")
![Western Blot: MDR1/ABCB1 Antibody (6G11F8) [NBP2-37290] - Western blot analysis using ABCB1 mAb against HEK293 (1) and ABCB1 (AA: 632-693)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11F8-Western-Blot-NBP2-37290-img0003.jpg)
![ELISA: MDR1/ABCB1 Antibody (6G11F8) [NBP2-37290] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-6G11F8-ELISA-NBP2-37290-img0001.jpg)
![Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Western blot analysis of extracts of various cell lines, using MDR1/ABCB1 antibody (NBP3-15660) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-2U4E9-Western-Blot-NBP3-15660-img0004.jpg "Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Western blot analysis of extracts of various cell lines, using MDR1/ABCB1 antibody (NBP3-15660) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: MDR1/ABCB1 Antibody (2U4E9) [NBP3-15660] - Western blot analysis of extracts of Rat liver, using MDR1/ABCB1 antibody (NBP3-15660) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-2U4E9-Western-Blot-NBP3-15660-img0005.jpg)
![Flow Cytometry: MDR1/ABCB1 Antibody (MRK16) - Chimeric [NBP2-52654] - Flow-cytometry using the MDR1/ABCB1 Antibody (MRK16) [NBP2-52654]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (NBP2-52654, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-MRK16-Chimeric-Flow-Cytometry-NBP2-52654-img0001.jpg "Flow Cytometry: MDR1/ABCB1 Antibody (MRK16) - Chimeric [NBP2-52654] - Flow-cytometry using the MDR1/ABCB1 Antibody (MRK16) [NBP2-52654]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (NBP2-52654, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunohistochemistry: MDR1/ABCB1 Antibody (A10F2-R) [NBP3-32687] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MDR1/ABCB1 antibody (NBP3-32687) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32687) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32687_mouse-mdr1-abcb1-mab-a10f2-r-29820241420975.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (A10F2-R) [NBP3-32687] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MDR1/ABCB1 antibody (NBP3-32687) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32687) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: MDR1/ABCB1 Antibody (A10E12-R) [NBP3-32685] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MDR1/ABCB1 antibody (NBP3-32685) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32685) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32685_mouse-mdr1-abcb1-mab-a10e12-r-298202414333680.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (A10E12-R) [NBP3-32685] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MDR1/ABCB1 antibody (NBP3-32685) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32685) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")