MAdCAM-1 Antibodies

HAF019). A specific band was detected for MAdCAM-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF019). A specific band was detected for MAdCAM-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAdCAM-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells of blood capillaries in intestinal villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells of blood capillaries in intestinal villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

993-MC) was serially diluted and captured by Rat Anti-Mouse MAdCAM‑1 Monoclonal Antibody (Catalog # MAB9931) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MAdCAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 993-MC) was serially diluted and captured by Rat Anti-Mouse MAdCAM‑1 Monoclonal Antibody (Catalog # MAB9931) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MAdCAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF018). A specific band was detected for MAdCAM‑1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for MAdCAM‑1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MAdCAM‑1 Monoclonal Antibody (Catalog # MAB6056) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for MAdCAM‑1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

993-MC) was serially diluted and captured by Rat Anti-Mouse MAdCAM‑1 Monoclonal Antibody (Catalog # MAB9931) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MAdCAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 993-MC) was serially diluted and captured by Rat Anti-Mouse MAdCAM‑1 Monoclonal Antibody (Catalog # MAB9931) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MAdCAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse MAdCAM‑1 Fc Chimera (Catalog # 993-MC) was serially diluted and captured by Rat Anti-Mouse MAdCAM‑1 Monoclonal Antibody (Catalog # MAB9931) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MAdCAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB60561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6056) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB60561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6056) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human MAdCAM-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human MAdCAM-1 Monoclonal Antibody (Catalog # MAB60561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6056) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Sheep Anti-Human MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6056) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Sheep Anti-Human MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6056) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human MAdCAM-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human MAdCAM-1 Monoclonal Antibody (Catalog # MAB60561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6056) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />