Anti-IL-6 Antibodies: Novus Biologicals

MAB206

IL-6 Antibody (6708) [Unconjugated]

(2 Reviews)

100 μg

MAB206

206-IL), Recombinant Mouse IL-6 (Catalog # 406-ML) and Recombinant Rat IL-6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/ Primate IL-6 Monoclonal Antibody (Catalog # MAB206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

Western blot shows 25 ng of Recombinant Human IL-6 (Catalog # <a class=

206-IL), Recombinant Mouse IL-6 (Catalog # 406-ML) and Recombinant Rat IL-6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/ Primate IL-6 Monoclonal Antibody (Catalog # MAB206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human IL-6 (Catalog # 206-IL), Recombinant Mouse IL-6 (Catalog # 406-ML) and Recombinant Rat IL-6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/ Primate IL-6 Monoclonal Antibody (Catalog # MAB206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

IL-6 was detected in immersion fixed frozen sections of hyperplastic human skin using Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206). The ND₅₀ is typically 8.00 - 80.0 ng/mL." class="big_lightbox">

Recombinant Human IL-6 (Catalog # <a class=

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206). The ND₅₀ is typically 8.00 - 80.0 ng/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>The role of IL‐6 signaling in the upregulation of PD‐L1 and downregulation of NKG2D ligands in CRPC cells. (A) PD‐L1 level in C4‐2siIL‐6/sc and CWRsiIL‐6/sc cell lines (left panel, mRNA level; right panel, protein level). (B) PD‐L1 IHC staining of tumor tissues. Error bars and significance values were obtained by counting positively stained cells in one randomly chosen phase of slides of three different stains. Magnification, 20× (inlet, 100×). (C) Blocking of IL‐6 Ab by neutralizing Ab of IL‐6 and the effect on PD‐L1 level in C4‐2sc and CWRsc cells. Cells were treated with either IL‐6 Ab or control IgG, total RNA extracted, cDNA converted, and the expression of PD‐L1 was compared in qPCR analyses. (D) PD‐L1 level in parental C4‐2 and CWR22Rv1 cells upon the addition of rhIL‐6. Parental cells (C4‐2P and CWR22Rv1P) were treated with rhIL‐6 (20 ng·mL−1) and PD‐L1 mRNA level was analyzed. (E) IHC staining of CRPC patient tumor samples. Two sets of adjacent tumor tissues (both samples, CRPC stage, Gleason score 8, patient age 70, Ningbo hospital in China) were stained with IL‐6 and PD‐L1. Arrows indicate the area showing positive staining of two molecules. (F) NKG2D ligand levels in IL‐6‐expressing cells and in IL‐6‐knockdown cells. Levels of five NKG2D ligands in C4‐2siIL‐6/sc and CWRsiIL‐6/sc cells were analyzed in qPCR analyses. (G) NKG2D ligand levels in parental C4‐2 and CWR22Rv1 cells upon the addition of rhIL‐6. Parental cells (C4‐2P and CWR22Rv1P) were treated with rhIL‐6 (20 ng·mL−1) and the NKG2D ligand levels (mRNA) were analyzed. (H) Flow cytometric analyses of NKG2D and PD‐1 on NK cells. Left two panels, primary NK cells were stained with PE‐NKG2D or APC‐PD‐1 and positive staining was analyzed. Right two panels, flow cytometric analyses of PD‐1 on NK cells, after coculture with tumor cells (6 h of incubation). Primary NK cells were added into tumor cells (1 : 1 ratio, tumor cells/NK cells) and collected after 6 h of incubation. PD‐1 levels in the collected

<b>Biological Strategies Validation. </b>Autocrine IL-6 activates STAT3 signalling in lung cancer cell-induced epidural ADSCs. a Epidural ADSCs were pre-treated with CM from lung cancer cells for 48 h, and pSTAT3 and STAT3 expression levels were detected by western blotting. Epidural ADSCs cultured in untreated medium served as a control. b The effects of lung cancer cell CM on epidural ADSC proliferation were evaluated using the CCK-8 assay. Epidural ADSCs were treated with CM from one of four lung cancer cell lines, and the optical density of both groups at 450 nm was analysed. Data from three separate experiments are shown. c Western blot analysis of pSTAT3 and STAT3 in epidural ADSCs treated with either 10 ng/mL recombinant IL-6, 10 ng/mL recombinant IL-11 or 50 ng/mL recombinant LIF in the presence or absence of either neutralizing antibodies or isotype controls. Loading control, actin. d Western blot analysis of pSTAT3 and alpha -SMA expression in epidural ADSCs treated with lung cancer cell CM in the presence or absence of neutralizing antibodies against IL-6, IL-11 or LIF. Loading control, actin. *P < 0.05; **P < 0.01; ***P < 0.001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31196220), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Activated ADSCs trigger the proliferation and invasion of lung cancer cells by regulating MMP2/9 expression and EMT. a The effects of activated ADSCs on lung cancer cell proliferation were evaluated using the CCK-8 assay. Four lung cancer cell lines were cultured in the presence of ADSC-CM or aADSC-CM, and the optical density at 450 nm was analysed. The cancer cells cultured alone in normal growth medium were used as negative controls. Data from three separate experiments are shown. b The number of lung cancer cells that migrated through 8-μm Transwell membrane pores was counted to determine the changes in the invasive capabilities in response to CM from epidural ADSCs or aADSCs. c MMP2/9, E-cadherin and vimentin expression levels in four lung cancer cell lines treated with ADSC-CM or aADSC-CM were examined by western blotting. Lung cancer cells cultured in an untreated medium served as controls. d Lung cancer cells were treated with neutralizing antibodies against IL-6, isotype controls and aADSC-CM. Lung cancer cell invasion was analysed using a Transwell assay. Lung cancer cells cultured with untreated medium served as negative controls. e Lung cancer cells were treated with neutralizing antibodies against IL-6, isotype controls and aADSC-CM. MMP2/9, E-cadherin and vimentin expression levels in four lung cancer cell lines were analysed using western blotting. Lung cancer cells cultured with untreated medium served as negative controls. ***P < 0.001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31196220), licensed under a CC-BY license. Not internally tested by R&D Systems.

Musashi-1 mitigated DDP-induced apoptosis via the IL-6/AKT regulatory loopA. Cells were pretreated with 50 μM of LY294002 or vehicle for 3 hours, followed by 50 μM DDP treatment for 24 hours. The culture media were collected and the concentrations of IL-6 was determined by ELISA. B. 05MG-FlagMSI1 cells were treated with/without 50 μM DDP for 24 hours in the absence or presence of recombinant IL-6 (10 ng/ml) or anti-IL-6 neutralizing antibody (0.1 μg/ml). The cell lysates were analyzed by Western blot. C-D. The bar diagrams represent the quantified ratio of p-AKT-308/AKT and p-AKT-473/AKT obtained by the Western blot in B. E. 05MG cells were treated with control (serum free medium, S.F.) or condition media from 05MG-FlagMSI1 cells (C.M.) in the presence or absence of 50 μM DDP, IL-6 (10 ng/ml), and anti-IL-6 antibody (0.1 μg/ml) for 24 hours. The cell lysates were analyzed by Western blot to assess the levels of p-AKT-308, p-AKT-473, and total AKT. F-G. The bar diagrams represent the quantified ratio of p-AKT-308/AKT and p-AKT-473/AKT obtained in E. H. 05MG cells were treated as described in E and analyzed by Western blot. I-J. The bar diagrams represent the quantified ratio of cleavage-caspase-3/actin and cleaved-PARP/actin obtained in H. Data represent the mean ± S.D. of two independent experiments performed in triplicate. * P<0.05 vs AKT or Actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27285760), licensed under a CC-BY license. Not internally tested by R&D Systems.

circCUL2 regulates the phenotypic plasticity of CAFs and promote PDAC progression by IL6. A Gene Set Enrichment Analysis (GSEA) of affected signatures in fibroblasts (circCUL2 vs. control). B GSEA plots for inflammatory CAF (iCAF) signatures in circCUL2 overexpression NFs from control. C-D qRT–PCR analysis of iCAF markers (IL6, TNF-alpha and IL1 alpha ) and myCAF marker (Acta2 and Axin2) expression in circCUL2 overexpression NFs. E Flow cytometric analysis of PDGFR alpha and alpha -SMA expression in circCUL2 overexpression NFs. F-H. Representative cytokine arrays for circCUL2-overexpression NFs and control (n = 3). arrows indicate the cytokines with significant changes, which were further confirmed by qRT–PCR and ELISA. I-L EdU assay (I), colony formation (J), Scratch wound healing assays (K) and transwell assays (L) of PANC-1 cells treated with conditioned medium circCUL2-overexpression NFs or anti-IL6. Scale bar, 100 μm. M western blot analysis of STAT3 and p-STAT3 in PANC-1 cells. Data are expressed as the mean ± SD of three independent experiments. **p < 0.01 and ***p < 0.001 (two-tailed Student t-tests) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35189958), licensed under a CC-BY license. Not internally tested by R&D Systems.

circCUL2-overexpression NFs promote PDAC progression in vivo. A Representative Bioluminescence images, lung and HE staining of lung tissue of mice 4 weeks after tail vein injection of luc-PANC-1 cell treated with conditioned medium as indicated (n = 8 per group). Scale bar, 100 μm. B Relative luminescence intensity in each group. C Histogram analysis of the metastatic nodules number in per lung. D lung metastasis rate of each group (Chi-square test). E-F Representative bioluminescence images and histogram analysis of luminescence intensity in each at day 30 are shown (n = 6). G Abdominal metastasis rate was calculated for indicated group (Chi-square test). H Representative images of orthotopic model in each group on which autopsy was performed. Red arrow indicated primary tumor; S, spleen; T, primary tumor; M, metastasis. I Images of PDX from 2 patients in 5 mice. (J) Tumor growth curves of indicated group (n = 5). K qRT–PCR analysis of circCUL2 levels in PDX of mice before and after treatment. L Representative images of IHC for PDGFR alpha and IL6. Scale bar, 100 μm. Data are expressed as the mean ± SD. **p < 0.01 and ***p < 0.001 (two-tailed Student t-tests) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35189958), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Allophycocyanin	FAB206A
Biotin	FAB206B
CoraFluor 1	FAB206CL1
DyLight 350	FAB206D
DyLight 405	FAB206E
DyLight 488	FAB206K
DyLight 550	FAB206L
DyLight 594	FAB206M
DyLight 650	FAB206W
DyLight 680	FAB206Y
DyLight 755	FAB206Z
FITC	FAB206F
HRP	FAB206H
Janelia Fluor 525	FAB206JF525
Janelia Fluor 549	FAB206I
Janelia Fluor 646	FAB206J
Janelia Fluor 669	FAB206JF669
PE	FAB206P
PerCP	FAB206C
mFluor Violet 450 SE	FAB206MFV450
mFluor Violet 500 SE	FAB206MFV500
mFluor Violet 610 SE	FAB206MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB206-SP
Carrier Free	MAB206-500

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NB600-1131

IL-6 Antibody - Azide and BSA Free

(1 Review)

(75 Publications)

0.2 ml

NB600-1131

Western Blot: IL-6 Antibody [NB600-1131] - Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band 21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate.

Western Blot of IL-6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker

Immunocytochemistry/ Immunofluorescence: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. a Human brain ECs were treated with glioma-CM for 24 h, & cell lysates were subjected to multiplex cytokine array analysis. Left, a representative blot. Right, quantified dot intensity of most significantly changed cytokines. b Human microvascular brain ECs were treated with glioma-CM that were harvested from different human glioma cells. Cell lysates were immunoblotted. c Human microvascular brain ECs & tumor-associated ECs isolated from different GBM patients were subjected to immunoblot analysis. d Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains & tumors were stained with anti-CD31, anti-IL-6, & anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal & tumor tissues. Bar represents 50 μm. Zoom-in factor: 4 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29422647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

IL-6 and IL-6 receptors are elevated in murine and human post thrombotic tissue. A. Plasma IL-6 levels drawn following sham surgery with retroperitoneal dissection or surgery with IVC ligation in WT mice resulting in stasis thrombosis (n = 5 per group). Following sham surgery or stasis thrombosis, vein wall lysate protein levels (B) of IL-6 (C) and CD126 (D) measured by Western blotting (n = 4–10/group). Gel blots cropped from different parts of the gel are presented, with white line separation. Protein levels of intrathrombus (E) IL-6 (F) and CD126 receptor (G) were measured at 4 and 8 days in partially occlusive (stenosis) and completely occlusive thrombi (stasis). All data presented as mean +/- standard deviation. Normally distributed data was compared utilizing unpaired student’s test with Welch’s correction and non-normally distributed data compared with Mann–Whitney U test. *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36828892), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Regulation of IL-6 by sorafenib was dosage and time specific (A-C). Low and median dosages of sorafenib (2.5-7.5 umol/L) upregulated IL-6 expression after 24 h to 72 h of administration in HCCLM3‑wt cells; high dosage of sorafenib (10 umol/L) upregulated IL-6 expression in 24 h but downregulated IL-6 expression from 48 h to 72 h of administration (Western blot analysis, *** P < 0.001); Regulation of PFKFB3 by sorafenib is dosage and time specific (D-F). Low and median dosages of sorafenib (2.5-7.5 umol/L) downregulated PFKFB3 expression in 24 h but upregulated it from 48 h to 72 h. A high dosage of sorafenib (10 umol/L) down-regulated PFKFB3 expression after 24 h to 72 h of administration in HCCLM3‑wt cells (Western blot analysis, *** P < 0.001); Regulation of HIF-1 alpha by sorafenib is dosage and time specific (G-I). Low and median dosage of sorafenib (2.5-7.5 umol/L) upregulated HIF-1 alpha expression in 24 h but downregulated it from 48 h to 72 h of administration. High dosage of sorafenib (10 umol/L) up-regulated PFKFB3 expression after 24 h to 72 h of administration in HCCLM3‑wt cells (Western blot analysis, *** P < 0.001) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37476196), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Hippocampal regulatory proteins for synaptic plasticity were restored, and inflammatory cytokines decreased in sleep-deprived mice treated with SS31. Western blotting was performed on hippocampus using specific antibodies for the detection of (A) NMDA receptor, (B) p-CREB, (C) BDNF, (D) MCP-1, (E) TNF-alpha , (F) IL-6, and (G) caspase-3, with Actin used as loading control. The densitometry values for the proteins were normalized to those of Actin, with non-SD group being 1. All data represent the mean +/- SEM (n = 5 per group). ** p < 0.01, *** p < 0.001, statistically significant difference between SD group/SD + Saline group and Non-SD group. # p < 0.05, ## p < 0.01, statistically significant difference between SD + SS31 group and SD group/SD + Saline group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33089207), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

A20 downregulation promoted necroptosis in vitro. (A) Primary neuron and astrocyte were obtained and verified in relative antibody. (B) A20 protein expression in primary neuron, primary astrocyte and HAPI. (C) Relative mRNA expression of A20 in different cell types was obtained from a database of Stanford University (//web.stanford.edu/group/barres_lab/brain_rnaseq.html). (D) A20 was significantly decreased after si-A20 treatment in the three cell types, including primary neuron, primary astrocyte and HAPI. (E,F) Tumor necrosis factor (TNF)-alpha (10 ng/ml) + Z-VAD (100 μm) combination (TZ) as a necroptosis inducer was used for 24 h. In primary neuron, RIP1, RIP3 and MLKL were tested by western blot. Data were analyzed by statistical. (G,H) TUNEL assays and (I) CCK-8 (n = 4) were used to test cell death and viability of neuron after 24 h treatment. (J–M) Western blot assay was used to test RIP1, RIP3, MLKL, NF-KB and relative inflammatory factors in primary HAPI and astrocyte respectively. Data were measured by one way ANOVA plus Tukey’s test. *P < 0.05 and **P < 0.01 vs. TZ group; #P < 0.05 and ##P < 0.01 vs. TZ+si-NC group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31607859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

AAV-shA20 restored inflammatory responses. (A,B) Interleukin-6 (IL-6), interleukin-1 (IL-1 beta ) and NLRP3 were detected in cortex tissues and hippocampal CA1 tissues by immunoblotting, respectively. The results including cortex (C–E) and hippocampus CA1 (F–H) were analyzed by statistical. (I) Representative images of double IL-1 beta (red) and Iba-1 (green) positive cells; NLRP3 (red) and Iba-1 (green); IL-6 (red) and GFAP (green) in the hippocampus CA1 location, as determined by immunofluorescence staining. Nuclei were stained with DAPI (blue). Data were measured by one way ANOVA plus Tukey’s test. *P < 0.05 and **P < 0.01 vs. CCI+AAV-shCtrl group. #P < 0.05 and ##P < 0.01 vs. CCI+AAV-shCtrl+Nec-1 or CCI+AAV-shCtrl+melatonin group, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31607859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effects of CpG ODN and fMLP stimulation on the levels of IL6 and TNF alpha in Z310 cells. Protein levels of IL6 and TNF alpha in Z310 cells treated with 2.5 uM of CpG ODN (TLR9 agonist) and 20 uM fMLP (FPR2 agonist) were analyzed using ICC and Western blots. Panel (A) shows the immunoquantification of IL6 and TNF alpha in cells treated with CpG ODN for 24 h. Results of densitometric measurements of bands from triplicate Western blot analysis normalized to the housekeeping protein vinculin are depicted in the graphs in panel (B) Graph (C) denotes the immunoquantification of IL6 and TNF alpha in cells treated with fMLP for 24 h. Densitometric measurements of bands from triplicate Western blot analysis normalized to the vinculin are shown in panel (D) graphs. Results are characterized as means +/- SEM. *Indicates p < 0.05, and **indicates p < 0.01 compared to the control. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fpain.2024.1488369/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Downstream receptor for advanced glycation end-products (RAGE) and nuclear factor (NF)-kappa B pathway were inhibited after treatment of Nec-1 and melatonin. NF-kappa B pathway was detected by immunoblotting in the cortex (A–E) and hippocampus CA1 (F–J). Relative downstream inflammatory factors were detected by immunoblotting in the cortex (K–N) and hippocampus CA1 (O–R). All experiments were performed in triplicate by one way ANOVA plus Tukey’s test. **P < 0.01 and ***P < 0.001 vs. sham group. #P < 0.05 and ##P < 0.01 vs. CCI group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31607859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NBP1-42761
HRP	NBP1-42762

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB600-1131SS

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AF-406-NA

IL-6 Antibody [Unconjugated]

(1 Review)

(53 Publications)

100 μg

AF-406-NA

HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

<b>Biological Strategies Validation. </b>IL-6 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA). The ND₅₀ is typically 0.01-0.03 µg/mL." class="big_lightbox">

Recombinant Mouse IL-6 (Catalog # <a class=

406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA). The ND₅₀ is typically 0.01-0.03 µg/mL." class="big_thumb" />

EphA2‐Dependent tumor induction of osteoclast differentiation requires IL‐6. (A) ELISA analysis for IL‐6 protein levels in conditioned media harvested from 4T1. delta C cells relative to 4T1.V controls (p < 0.05, Mann–Whitney test). (B) Graph shows the % tartrate‐resistant acid phosphatase (TRAP)+ osteoclasts in indirect cocultures of osteoclast progenitors with 4T1.V + IgG, 4T1.V + anti‐mouse IL‐6 neutralizing antibody, 4T1. delta C + PBS, and 4T1. delta C + recombinant murine IL‐6. (p < 0.05, Mann–Whitney test). (C) Graph shows the % TRAP+ osteoclasts in direct cocultures of osteoclast progenitors with 4T1.V + IgG, 4T1.V + anti‐mouse IL‐6 neutralizing antibody, 4T1. delta C + PBS, and 4T1. delta C + recombinant murine IL‐6, (p < 0.05, Mann–Whitney test). There were five to eight fields per condition from three independent experiments. (D) Immunoblots show phosphorylated Stat3 levels in osteoclast progenitor cells treated with IL‐6 in the presence or absence of neutralizing anti‐mouse IL‐6 antibody. Uniform loading was confirmed by probing blots for total Stat3 and actin. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33869989), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inflammatory cytokines. (a) Representative band image and Coomassie brilliant blue staining of the total amount of protein; (b) TNF‐ alpha ; (c) IL‐6; and (d) IL‐1 beta . The data are present in each plot as the mean ± standard deviation (n = 6 per group). dpi, days post‐injury; DW, distilled water group; SP, spermidine group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39448391), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inflammatory cytokines. (a) Representative images of the bands and Coomassie brilliant blue staining of the total amount of protein; (b) TNF‐ alpha ; (c) IL‐6. The data are presented as the mean ± standard deviation (n = 6 per group). DW, Distilled water group; SP, Spermidine group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39910742), licensed under a CC-BY license. Not internally tested by R&D Systems.

VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

IL‑6 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Goat Anti-Mouse IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-406-NAU-100UG
Alexa Fluor 405	AF-406-NAV-100UG
Alexa Fluor 488	AF-406-NAG-100UG
Alexa Fluor 532	AF-406-NAX-100UG
Alexa Fluor 594	AF-406-NAT-100UG
Alexa Fluor 647	AF-406-NAR-100UG
Alexa Fluor 700	AF-406-NAN-100UG
Alexa Fluor 750	AF-406-NAS-100UG
Alexa Fluor Plus 405	AF-406-NAAFP405
Alexa Fluor Plus 488	AF-406-NAAFP488
Alexa Fluor Plus 555	AF-406-NAAFP555
Alexa Fluor Plus 594	AF-406-NAAFP594
Alexa Fluor Plus 647	AF-406-NAAFP647
Alexa Fluor Plus 680	AF-406-NAAFP680
Allophycocyanin	AF-406-NAA
Biotin	BAF406
CoraFluor 1	AF-406-NACL1
DyLight 350	AF-406-NAD
DyLight 405	AF-406-NAE
DyLight 488	AF-406-NAK
DyLight 550	AF-406-NAL
DyLight 594	AF-406-NAM
DyLight 650	AF-406-NAW
DyLight 680	AF-406-NAY
DyLight 755	AF-406-NAZ
FITC	AF-406-NAF
HRP	AF-406-NAH
Janelia Fluor 525	AF-406-NAJF525
Janelia Fluor 549	AF-406-NAI
Janelia Fluor 646	AF-406-NAJ
Janelia Fluor 669	AF-406-NAJF669
PE	AF-406-NAP
PerCP	AF-406-NAC
mFluor Violet 450 SE	AF-406-NAMFV450
mFluor Violet 500 SE	AF-406-NAMFV500
mFluor Violet 610 SE	AF-406-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-406-SP

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AF506

IL-6 Antibody [Unconjugated]

(1 Review)

(54 Publications)

100 μg

AF506

HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Biological Strategies Validation. </b>IL‑6 was detected in immersion fixed NR8383 rat alveolar macrophage cell line using Goat Anti-Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cyoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

506-RL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-6 (0.6 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506). The ND₅₀ is typically <0.5 µg/mL." class="big_lightbox">

Recombinant Rat IL-6 (Catalog # <a class=

506-RL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-6 (0.6 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506). The ND₅₀ is typically <0.5 µg/mL." class="big_thumb" />

Diverse localization of measured cytokines in P7 brains. Representative pictures (100× magnification) of immunohistochemical stainings of IL-1 beta (Α), IL-10 (B), IL-6 (C) and TNF-alpha (D). Cytokines are stained with DAB (brown color) and counterstained with cresyl violet. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23351591), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF506U-100UG
Alexa Fluor 405	AF506V-100UG
Alexa Fluor 488	AF506G-100UG
Alexa Fluor 532	AF506X-100UG
Alexa Fluor 594	AF506T-100UG
Alexa Fluor 647	AF506R-100UG
Alexa Fluor 700	AF506N-100UG
Alexa Fluor 750	AF506S-100UG
Alexa Fluor Plus 405	AF506AFP405-100UG
Alexa Fluor Plus 488	AF506AFP488-100UG
Alexa Fluor Plus 555	AF506AFP555-100UG
Alexa Fluor Plus 594	AF506AFP594-100UG
Alexa Fluor Plus 647	AF506AFP647-100UG
Alexa Fluor Plus 680	AF506AFP680-100UG
Allophycocyanin	AF506A
Biotin	BAF506
CoraFluor 1	AF506CL1
DyLight 350	AF506D
DyLight 405	AF506E
DyLight 488	AF506K
DyLight 550	AF506L
DyLight 594	AF506M
DyLight 650	AF506W
DyLight 680	AF506Y
DyLight 755	AF506Z
FITC	AF506F
HRP	AF506H
Janelia Fluor 525	AF506JF525
Janelia Fluor 549	AF506I
Janelia Fluor 646	AF506J
Janelia Fluor 669	AF506JF669
PE	AF506P
PerCP	AF506C
mFluor Violet 450 SE	AF506MFV450
mFluor Violet 500 SE	AF506MFV500
mFluor Violet 610 SE	AF506MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF506-SP

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AF-206-NA

IL-6 Antibody [Unconjugated]

(4 Reviews)

(52 Publications)

100 μg

AF-206-NA

NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>IL-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND₅₀ is typically ≤ 125 ng/mL." class="big_lightbox">

<b>Biological Strategies Validation. </b>Recombinant Human IL-6 (Catalog # <a class=

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND₅₀ is typically ≤ 125 ng/mL." class="big_thumb" />

Breast DCIS cells overexpress proinflammatory markers. Representative images of immunohistochemistry targeting IL-6 protein in normal breast tissue (a), and breast DCIS (b) (N = 61). c and d Hematoxylin staining of serial sections from tissue shown in panel A and B. All images are 20X magnification. Scale bar equals 100 μm. e Evaluation of IL-6 gene expression in DCIS cells via qRT-PCR; the isogenic MCF10.DCIS cells and the non-isogenic SUM102 cell line were analyzed against the non-transformed MCF10A cell line (N = 3). f Secretion of IL-6 protein from DCIS cell lines and non-transformed MCF10A cells as determined by ELISA. *P < 0.05, Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>IL-6 neutralizing antibody (nAb) inhibits growth of MCF10.DCIS structures. Representative contiguous 16-tiled DIC images of MCF10.DCIS cells grown in MAME cultures for 8 days. MAME culture of MCF10.DCIS cells treated with an isotype-matched antibody against IgG (control) (8 days) (a), or 1 μg /ml IL-6 nAb (b) (8 days). MCF10.DCIS cells treated with IL-6 nAb followed by a 48 h treatment-free recovery period prior to imaging on day 10 (c). N = 3, Scale bars, 200 μm. d Diameter of MCF10.DCIS structures in the presence of control antibody, IL-6 nAb, or 48 h recovery from IL-6 nAb. N = 20-40 measurements /tiled DIC image (N = 3). ****P ≤ 0.0001, **P ≤ 0.01. e Evaluation of gene expression of invasive tumor cell markers in MAME cultures treated with IL-6 nAb (N = 3). Fold difference as compared to control cultures. Dashed line indicates 2-fold threshold. Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL-6 nAb inhibits CAF-stimulated MCF10.DCIS structure growth and results in altered morphology. Contiguous 16-tile DIC image of CAFs: WS12Ti (A, B) or CAF40TKi (c, d) co-cultured with MCF10.DCIS cells for 8 days in the presence of isotype matched anti-IgG (a, c) or 1 μg /ml IL-6 nAb (b, d). e Perimeter measurements from DCIS structures in MAME culture in the presence of anti-IgG or IL-6 nAb (N = 100-140 measurements from 6 contiguous tiled DIC images (N = 3). Fluorescent tiled images of CAF40TKi fibroblasts (unlabeled) cultured with MCF10.DCIS-RFP cells in the presence of anti-IgG (f) or 1 μg /ml IL-6 nAb (g). h Quantification of total volume of DCIS-RFP structures in co-culture with CAF40TKi fibroblasts (ratio-paired t-test, N = 3). ***P ≤ 0.001, **P ≤ 0.01; Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-206-NAU-100UG
Alexa Fluor 405	AF-206-NAV-100UG
Alexa Fluor 488	AF-206-NAG-100UG
Alexa Fluor 532	AF-206-NAX-100UG
Alexa Fluor 594	AF-206-NAT-100UG
Alexa Fluor 647	AF-206-NAR-100UG
Alexa Fluor 700	AF-206-NAN-100UG
Alexa Fluor 750	AF-206-NAS-100UG
Alexa Fluor Plus 405	AF-206-NAAFP405
Alexa Fluor Plus 488	AF-206-NAAFP488
Alexa Fluor Plus 555	AF-206-NAAFP555
Alexa Fluor Plus 594	AF-206-NAAFP594
Alexa Fluor Plus 647	AF-206-NAAFP647
Alexa Fluor Plus 680	AF-206-NAAFP680
Allophycocyanin	AF-206-NAA
Biotin	BAF206
CoraFluor 1	AF-206-NACL1
DyLight 350	AF-206-NAD
DyLight 405	AF-206-NAE
DyLight 488	AF-206-NAK
DyLight 550	AF-206-NAL
DyLight 594	AF-206-NAM
DyLight 650	AF-206-NAW
DyLight 680	AF-206-NAY
DyLight 755	AF-206-NAZ
FITC	AF-206-NAF
HRP	AF-206-NAH
Janelia Fluor 525	AF-206-NAJF525
Janelia Fluor 549	AF-206-NAI
Janelia Fluor 646	AF-206-NAJ
Janelia Fluor 669	AF-206-NAJF669
PE	AF-206-NAP
PerCP	AF-206-NAC
mFluor Violet 450 SE	AF-206-NAMFV450
mFluor Violet 500 SE	AF-206-NAMFV500
mFluor Violet 610 SE	AF-206-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-206-SP

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MAB2061

IL-6 Antibody (1936) [Unconjugated]

(53 Publications)

100 μg

MAB2061

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

IL-6 was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL-6 was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

<b>Biological Strategies Validation. </b>Human peripheral blood mononuclear cells (PBMCs) treated with 100 ng/mL LPS for 24 hours were stained with Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061, filled histogram) or isotype control antibody (<a class=

MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061). The ND₅₀ is typically 8.00-80.0 ng/mL." class="big_lightbox">

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061). The ND₅₀ is typically 8.00-80.0 ng/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

IL‑6 was detected in immersion fixed Human PBMCs stimulated with LPS (positive control) and absent in untreated human PBMCs (negative control) using Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules." class="big_lightbox">

PBMCs stimulated with 100 ng/ml LPS and Brefeldin A for 24 hrs (A) or unstimulated (B) were stained with Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # <a class=

FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Effects of topo I-reactive B cells on the differentiation of CD4+ T cells in systemic sclerosis (SSc) patients.B cells with low affinity for topo I and those with high affinity for topo I as well as topo I-non-reactive B cells were obtained from anti-topo I antibody-positive SSc patients (n = 111). These B cells were co-cultured with CD4+ T cells. After 48 hr of co-culture, mRNA was extracted from these cells and FoxP3 and ROR gamma t expression levels were examined by real-time RT-PCR. The results were presented when 96-well plates were used as a co-culture site (A) and when microculture plates (B) were used (C). These cells were further co-cultured on microculture plates, and the protein expression of CD4, CD20, FoxP3, and ROR gamma t was confirmed by fluorescent cell staining and signal intensity was determined by ImageJ (D). Similarly, co-culture in microculture plates in the presence of anti-IL-10 (10 μg/ml), anti-IL-35 (5 μg/ml), anti-IL-6 (1 μg/ml), or anti-IL-23 (5 μg/ml) antibodies (Abs) was conducted, followed by fluorescent cell staining (E). These results represented seven experiments. The bar graphs show the mean + SD. Original magnification, ×1000. *p<0.05.Figure 3—source data 1.Source file for the effects of topo I-reactive B cells on the differentiation of CD4+ T cells in systemic sclerosis (SSc) patients.This archive contains all data used for the quantitative analysis shown in Figure 3.Source file for the effects of topo I-reactive B cells on the differentiation of CD4+ T cells in systemic sclerosis (SSc) patients.This archive contains all data used for the quantitative analysis shown in Figure 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34854378), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC2061U-100UG
Alexa Fluor 405	IC2061V-100UG
Alexa Fluor 488	IC2061G-100UG
Alexa Fluor 532	FAB2061X
Alexa Fluor 594	IC2061T-100UG
Alexa Fluor 647	IC2061R-100UG
Alexa Fluor 700	IC2061N-100UG
Alexa Fluor 750	IC2061S-100UG
Alexa Fluor Plus 405	FAB2061AFP405-100UG
Alexa Fluor Plus 488	FAB2061AFP488-100UG
Alexa Fluor Plus 555	FAB2061AFP555-100UG
Alexa Fluor Plus 594	FAB2061AFP594-100UG
Alexa Fluor Plus 647	FAB2061AFP647-100UG
Alexa Fluor Plus 680	FAB2061AFP680-100UG
Allophycocyanin	FAB2061A
Biotin	FAB2061B
CoraFluor 1	FAB2061CL1
DyLight 350	FAB2061D
DyLight 405	FAB2061E
DyLight 488	FAB2061K
DyLight 550	FAB2061L
DyLight 594	FAB2061M
DyLight 650	FAB2061W
DyLight 680	FAB2061Y
DyLight 755	FAB2061Z
Fluorescein	IC206F
HRP	FAB2061H
Janelia Fluor 525	FAB2061JF525
Janelia Fluor 549	FAB2061I
Janelia Fluor 646	FAB2061J
Janelia Fluor 669	FAB2061JF669
PerCP	FAB2061C
Phycoerythrin	IC206P
mFluor Violet 450 SE	FAB2061MFV450
mFluor Violet 500 SE	FAB2061MFV500
mFluor Violet 610 SE	FAB2061MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC206F
Carrier Free	IC206P
Carrier Free	MAB2061-SP
Carrier Free	MAB2061-500
Carrier Free	IC2061V-100UG
Carrier Free	IC2061U-100UG
Carrier Free	IC2061T-100UG
Carrier Free	IC2061S-100UG
Carrier Free	IC2061R-100UG
Carrier Free	IC2061N-100UG
Carrier Free	IC2061G-100UG

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NBP2-16957

IL-6 Antibody - BSA Free

(1 Review)

(6 Publications)

0.1 ml

NBP2-16957

Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded human lung cancer.</br>IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (-) and treated (+) THP-1 whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (NBP2-16957) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (NBP2-16957) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, RM

Species Glossary

Reactivity: Hu, Mu, Rt, RM

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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AB-206-NA

IL-6 Antibody [Unconjugated]

(17 Publications)

1 mg

AB-206-NA

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Polyclonal Antibody (Catalog # AB-206-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_lightbox">

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Polyclonal Antibody (Catalog # AB-206-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." alt="Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Polyclonal Antibody (Catalog # AB-206-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows PBMC conditioned media untreated (-) or treated (+) with PHA , loaded at 0.2 mg/mL. A specific band was detected for IL‑6 at approximately 31 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IL‑6 Polyclonal Antibody (Catalog # AB-206-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-47810

IL-6 Antibody (OTI3G9)

(1 Review)

(2 Publications)

0.1 ml

NBP1-47810

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human prostate tissue using anti-IL6 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IL6 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human breast tissue using anti-IL6 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human colon tissue using anti-IL6 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human Kidney tissue using anti-IL6 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)

Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71027AF350
Alexa Fluor 405	NBP2-71027AF405
Alexa Fluor 488	NBP2-71027AF488
Alexa Fluor 532	NBP2-71027AF532
Alexa Fluor 594	NBP2-71027AF594
Alexa Fluor 647	NBP2-71027AF647
Alexa Fluor 700	NBP2-71027AF700
Alexa Fluor 750	NBP2-71027AF750
Alexa Fluor Plus 405	NBP2-71027AFP405
Alexa Fluor Plus 488	NBP2-71027AFP488
Alexa Fluor Plus 555	NBP2-71027AFP555
Alexa Fluor Plus 594	NBP2-71027AFP594
Alexa Fluor Plus 647	NBP2-71027AFP647
Alexa Fluor Plus 680	NBP2-71027AFP680
Allophycocyanin	NBP2-71027APC
Biotin	NBP2-71027B
CoraFluor 1	NBP2-71027CL1
DyLight 350	NBP2-71027UV
DyLight 405	NBP2-71027V
DyLight 488	NBP2-71027G
DyLight 550	NBP2-71027R
DyLight 594	NBP2-71027DL594
DyLight 650	NBP2-71027C
DyLight 680	NBP2-71027FR
DyLight 755	NBP2-71027IR
FITC	NBP2-71027F
HRP	NBP2-71027H
Janelia Fluor 525	NBP2-71027JF525
Janelia Fluor 549	NBP2-71027JF549
Janelia Fluor 646	NBP2-71027JF646
Janelia Fluor 669	NBP2-71027JF669
PE	NBP2-71027PE
PerCP	NBP2-71027PCP
mFluor Violet 450 SE	NBP2-71027MFV450
mFluor Violet 500 SE	NBP2-71027MFV500
mFluor Violet 610 SE	NBP2-71027MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71027

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AF686

IL-6 Antibody [Unconjugated]

(9 Publications)

100 μg

AF686

686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND₅₀ is typically 0.15-0.6 µg/mL." class="big_lightbox">

Recombinant Porcine IL‑6 (Catalog # <a class=

686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND₅₀ is typically 0.15-0.6 µg/mL." alt="Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND₅₀ is typically 0.15-0.6 µg/mL." class="big_thumb" />

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF686U-100UG
Alexa Fluor 405	AF686V-100UG
Alexa Fluor 488	AF686G-100UG
Alexa Fluor 532	AF686X-100UG
Alexa Fluor 594	AF686T-100UG
Alexa Fluor 647	AF686R-100UG
Alexa Fluor 700	AF686N-100UG
Alexa Fluor 750	AF686S-100UG
Alexa Fluor Plus 405	AF686AFP405-100UG
Alexa Fluor Plus 488	AF686AFP488-100UG
Alexa Fluor Plus 555	AF686AFP555-100UG
Alexa Fluor Plus 594	AF686AFP594-100UG
Alexa Fluor Plus 647	AF686AFP647-100UG
Alexa Fluor Plus 680	AF686AFP680-100UG
Allophycocyanin	AF686A
Biotin	BAF686
CoraFluor 1	AF686CL1
DyLight 350	AF686D
DyLight 405	AF686E
DyLight 488	AF686K
DyLight 550	AF686L
DyLight 594	AF686M
DyLight 650	AF686W
DyLight 680	AF686Y
DyLight 755	AF686Z
FITC	AF686F
HRP	AF686H
Janelia Fluor 525	AF686JF525
Janelia Fluor 549	AF686I
Janelia Fluor 646	AF686J
Janelia Fluor 669	AF686JF669
PE	AF686P
PerCP	AF686C
mFluor Violet 450 SE	AF686MFV450
mFluor Violet 500 SE	AF686MFV500
mFluor Violet 610 SE	AF686MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF686-SP

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AB-406-NA

IL-6 Antibody [Unconjugated]

(9 Publications)

1 mg

AB-406-NA

406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Polyclonal Antibody (Catalog # AB-406-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_lightbox">

406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Polyclonal Antibody (Catalog # AB-406-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." alt="Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Polyclonal Antibody (Catalog # AB-406-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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MAB686

IL-6 Antibody (77830)

(8 Publications)

500 μg

MAB686

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

IL-6 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB686) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-6 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB686) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB686U-100UG
Alexa Fluor 405	FAB686V-100UG
Alexa Fluor 488	FAB686G-100UG
Alexa Fluor 532	FAB686X-100UG
Alexa Fluor 594	FAB686T-100UG
Alexa Fluor 647	FAB686R-100UG
Alexa Fluor 700	FAB686N-100UG
Alexa Fluor 750	FAB686S-100UG
Alexa Fluor Plus 405	FAB686AFP405-100UG
Alexa Fluor Plus 488	FAB686AFP488-100UG
Alexa Fluor Plus 555	FAB686AFP555-100UG
Alexa Fluor Plus 594	FAB686AFP594-100UG
Alexa Fluor Plus 647	FAB686AFP647-100UG
Alexa Fluor Plus 680	FAB686AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB686-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

IL-6 Antibodies

IL-6 Antibody (6708) [Unconjugated]

IL-6 Antibody - Azide and BSA Free

IL-6 Antibody [Unconjugated]

IL-6 Antibody [Unconjugated]

IL-6 Antibody [Unconjugated]

IL-6 Antibody (1936) [Unconjugated]

IL-6 Antibody - BSA Free

IL-6 Antibody [Unconjugated]

IL-6 Antibody (OTI3G9)

IL-6 Antibody [Unconjugated]

IL-6 Antibody [Unconjugated]

IL-6 Antibody (77830)