MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB530-SP | |||
AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " alt="Mouse Splenocytes were stained with Goat Anti-Mouse IL-4R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF530, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB530P-100 | |||
| Carrier Free | FAB530P-025 | |||
| Carrier Free | AF530-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-230-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 230-4R-025/CF | |||
204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-4 Ra Polyclonal Antibody (Catalog # AB-230-NA). The ND50 is typically 5-10 µg/mL." alt="Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-4 Ra Polyclonal Antibody (Catalog # AB-230-NA). The ND50 is typically 5-10 µg/mL." class="big_thumb" />
CP004), the concentration of Recombinant Human IL-4 (Catalog # 204-IL) that produces 50% of the optimal binding response is approximately 3-20 ng/mL." alt="When Biotinylated Recombinant Human IL-4R alpha Fc Chimera Avi-tag (Catalog # AVI7700) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004), the concentration of Recombinant Human IL-4 (Catalog # 204-IL) that produces 50% of the optimal binding response is approximately 3-20 ng/mL." class="big_thumb" />
204-IL/CF) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-4R alpha His-tag Avi-tag Protein (Catalog # AVI11165) binds with an ED50 of 70.0-560 ng/mL." alt="When Recombinant Human IL-4 (204-IL/CF) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-4R alpha His-tag Avi-tag Protein (Catalog # AVI11165) binds with an ED50 of 70.0-560 ng/mL." class="big_thumb" />
CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for 
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 Ra Monoclonal Antibody (Catalog # MAB230). The ND50 is typically 3-6 ng/mL." class="big_thumb" />
![In vitro binding assays of [V14]6 and [AP1-V12]6 polymer.(A, B) H226, (C, D) MDA-MB-231 and (E, F) H460 cells were incubated with 10 µM of [V14]6, [AP1-V12]6 and AP1 for 1 h at 4°C. Cell binding was determined using flow cytometry. Histograms are representative of three independent experiments. Graphical bars (on right) represent the percent of Alexa 488 labeled polymer bound to cells as mean ±SD of data obtained from three separate experiments performed in triplicates. ***P<0.0001, **P<0.001, and *P<0.05, one-way ANOVA; n = 3. (G, H) H226 cells (3×105 cells) were pre- incubated with different concentrations (1, 5 and 10 µg/mL) of anti-IL-4 receptor antibody followed by 1 h incubation with 10 µM Alexa-labeled [AP1-V12]6 at 4°C. The cells were further suspended in 300 µL of PBS after washing and analyzed using flow cytometry. Histograms are representative of three independent experiments. Graphical bars represent the percent of Alexa 488 labeled polymer bound to cells as mean ±SD of data obtained from three separate experiments performed in triplicates. ***P<0.0001, One way ANOVA; n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081891), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab230_human-il-4-r-alpha-mab-clone-25463-4120241048249.jpg)
![In vivo, ex vivo imaging and biodistribution of [AP1-V12]6 polymers.(A) Cy5.5-labeled [V14]6 and [AP1-V12]6 (8 mg/kg) were intravenously injected into MDA-MB-231 tumor xenografted nude mice. Biodistribution was determined by collecting in vivo fluorescence images at different time points. Scale bar indicates normalized fluorescence intensity (NC). Representative optical images of three experiments. (B) Quantitation of fluorescence intensities in tumor sites at respective time points (n = 3). (C) Analysis of fluorescence intensities for tumors and organs from ex vivo images (n = 3). (D) Histological analysis of [AP1-V12]6 polymer (red) localization in tumors. Nuclei were stained with DAPI (blue), and IL-4R expression on cells was visualized by anti-IL-4 receptor antibody staining (green). Representative confocal images of three experiments (scale bar 50 µm). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081891), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab230_human-il-4-r-alpha-mab-clone-25463-41202410485426.jpg)
