GFP Antibodies
We offer GFP antibodies that have been cited in 350+ publications with 30+ customer reviews to detect the expression of GFP fusion proteins. Browse our extensive catalog for polyclonal, monoclonal, and conjugated anti-GFP antibodies. Validated research applications for GFP antibodies include ELISA, Electron Microscopy, Flow Cytometry, CyTOF-ready, ICC, Immunofluorescence, IHC, IHC-Frozen, IHC-Paraffin, IHC-Whole Mount, IP, Simple Western, and Western Blot. Choose the anti-GFP antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each GFP antibody is fully covered by our Guarantee+.
Alternate Names for GFP Antibodies
anti-GFP antibody, anti-Enhanced Green Fluorescent Protein antibody, anti-green fluorescent protein (gfp) antibody, anti-Green Fluorescent Protein antibody
2 Results for "gfp" in Primary Antibodies
![Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye(R) 800 conjugated Donkey-a-Goat IgG [H&L] MX7 () secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-25520231310522.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Viral recombination of Oxtr in anterior DG hilar neurons impairs discrimination of social, but not non-social, stimuli. a Schematic illustrating viral injection & behavioral testing timeline. b Representative images of Cre & GFP virus infection in aDG. Scale bar, 200 μm. c Representative images & quantifications of cFos immunoreactivity in granule cell layer of aDG (GFP: n = 7, Cre: n = 4). Scale bar, 75 μm. d Behavioral schematic (top) & quantification (bottom) of single object exploration (GFP: n = 11, Cre: n = 6). e Behavioral schematic (top) & quantification (bottom) of novel objection recognition (GFP: n = 11, Cre: n = 6). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). f Behavioral schematic (top) & quantification (bottom) of social exploration test (GFP: n = 11, Cre: n = 6). g Behavioral schematic (top) & quantification (bottom) of social discrimination task (GFP: n = 11, Cre: n = 6). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). All data are displayed as mean ± SEM Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29222469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-310202415525264.jpg)
![Immunohistochemistry: GFP Antibody [NB100-1770] - Macrophages regulate dormancy in tumor cells.a Representative image of triple immunofluorescently stained in E0771-GFP primary tumor tissue for tumor cells, macrophages, & NR2F1. Green = GFP; Red = NR2F1; White = IBA-1; Blue = DAPI. White arrow shows a macrophage. The yellow arrow contact between an NR2F1-positive tumor cell & a macrophage. Mϕ=Macrophage. Scale bar=20 μm. b Quantification showing frequency of distances between NR2F1+ tumor cells to nearest macrophage in primary tumor. Data is normalized to frequency of distances between all DAPI+ nuclei to nearest TMEM. Bar = mean. Error bars = ±SEM. n = 34 fields of view (551 × 316 µm2) in 4 animals. For comparison between 0 & 200 µm bins a two-tailed Mann-Whitney test used (p < 0.0001). ****p < 0.0001. c Representative immunofluorescence images of NR2F1 expression in E0771-GFP tumor cells cultured alone, in direct contact w/ BAC1.2F5 macrophages, or in direct contact w/ HUVEC endothelial cells. White arrows show macrophages or endothelial cells in direct contact w/ a tumor cell. Green = GFP; Red = NR2F1; Blue = DAPI. TC = Tumor Cell. Mϕ = Macrophage. EC = Endothelial Cell. Scale bar = 15 μm. d Percentage of NR2F1-positive tumor cells from each group in C. TC alone: n = 777 cells in 9 independent experiments; TC+Mϕ; n = 226 cells in 6 independent experiments, TC+EC = n = 359 cells in 4 independent experiments. Bar = mean. Error bars = ±SEM. For TC vs. TC+Mϕ (p = 0.0039), & for TC vs. TC+EC (p = 1), a two-tailed Kruskal-Wallis test w/ Dunn’s multiple comparisons adjustment used. For TC+Mϕ vs. TC+EC (0.012), a two-tailed one-way ANOVA w/ Sidak’s multiple comparison adjustment used. *p < 0.05. **p < 0.01; ns = not significant. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/35110548), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-31020241553380.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Viral recombination of Oxtr in aCA2/CA3distal impairs discrimination of social, but not non-social, stimuli. a Schematic illustrating viral injection & behavioral testing timeline. b Representative images of Cre & GFP virus infection in aCA2/CA3. Scale bar, 200 μm. c Behavioral schematic (top) & quantification (bottom) of single object exploration (GFP: n = 7, Cre: n = 10). d Behavioral schematic (top) & quantification (bottom) of novel objection recognition (GFP: n = 7, Cre: n = 10). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). e Behavioral schematic (top) & quantification (bottom) of social exploration test (GFP: n = 7, Cre: n = 10). f Behavioral schematic (top) & quantification (bottom) of social discrimination task (GFP: n = 7, Cre: n = 10). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). All data are displayed as mean ± SEM Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29222469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-31020241682115.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Transduction of mature retinal ganglion cells.Dissociated retinal ganglion cells (RGCs) isolated from adult rats (a–c), mice (d–f) & zebrafish (g–i) were transduced with baculovirus (bv) encoding fGFP (rat & mice) or DsRed (zebrafish). Representative pictures of vehicle- (-) & bv -treated cultures (a,d,g) visualize transduced RGCs (GFP & DsRed, respectively) that were either co-stained with the neuronal marker beta III-tubulin (Tub, red) at 2 days after transduction (d.a.t) for rat & mice RGCs (a,d) or identified by EGFP expression for zebrafish RGCs at 6 d.a.t. (g). The percentage of transduced RGCs was determined at 1, 2 & 3 days after transduction (d.a.t.) for rat & mice (b,e) & at 4 & 6 d.a.t. for zebrafish (h). Treatment effects compared to vehicle-treated controls: ***p < 0.001, **p < 0.01. Scale bars: 50 μm. RGC survival (c,f,i) was not affected by baculovirus application (bv) compared to vehicle-treated controls (-). (j) Delayed transduction of adult zebrafish RGCs with DsRed-bv after 4 days in culture. Scale bar: 50 μm. (k,l) Co-transduction of adult rat RGCs with fGFP-bv & DsRed-bv. The two viruses were added to retinal cultures simultaneously at half the concentration of single transductions. Representative pictures show co-transduced, fGFP- & DsRed-expressing RGCs (green & red, respectively) that were co-stained against the neuronal marker beta III-tubulin (white) at 2 d.a.t. Scale bar: 50 μm. (k). The percentage of transduced RGCs was determined at 2 & 3 d.a.t. (l). Non-significant difference in co-tranduction efficiency compared to single transductions. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep38928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-31020241612616.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Transduction of dorsal root ganglion neurons.Dissociated dorsal root ganglion neurons (DRG) isolated from postnatal (a–c) or adult (d–f) rats & adult mice (g–i) were transduced with fGFP-baculovirus (bv). Representative pictures of vehicle (-) & bv -treated cultures (a,d,g) show transduced, GFP-expressing neurons (green) that were co-stained with the neuronal marker beta III-tubulin (Tub, red) at 2 days after transduction (d.a.t.). The percentage of transduced, GFP-expressing DRG neurons was determined at 1, 2 & 3 d.a.t. (b,e,h), revealing similar transduction efficiencies of 80–90% in postnatal & adult DRG neurons. Cell survival in postnatal (c) & adult (f, i) DRG cultures was not affected by bv-application compared to vehicle-treated controls (-). Scale bars: 100 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep38928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-310202415525262.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - hnRNPA3 knockout enhances DNA damage in DPR expressing HeLa cells. a Immunocytochemical detection of gamma H2AX foci in GFP-tagged DPR-expressing HeLa WT & A3KO HeLa cells. b Fold change of gamma H2AX foci in HeLa WT & A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 47–127 cells from 2 biological replicates. c Immunocytochemical detection of pATM foci in GFP-tagged DPR-expressing HeLa WT & A3KO HeLa cells. d Fold change of pATM foci in HeLa WT & A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 71–263 cells from 2 biological replicates. GFP EGFP transfected, GA EGFP-tagged poly-GA 175 repeats transfected, GR EGFP-tagged poly-GR 177 repeats transfected, PR: EGFP-tagged poly-PR 176 repeats transfected. All graphs are shown as mean ± SEM. *p < 0.05, **p < 0.01; one-way ANOVA & Tukey’s post-hoc test. Scale bar 10 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31642962), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-310202415541948.jpg)
