eIF2A Antibodies
We offer eIF2A Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each eIF2A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our eIF2A Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the eIF2A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our eIF2A polyclonal antibodies and browse our eIF2A monoclonal antibody catalog.
Alternate Names for eIF2A Antibodies
anti-eIF2A antibody, anti-EIF-2 antibody, anti-EIF-2A antibody, anti-EIF-2alpha antibody, anti-EIF2AEIF2 antibody, anti-eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa antibody, anti-subunit 1 (alpha, 35kD ) antibody
8 Results for "eif2a" in Primary Antibodies
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0011.jpg "Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Staining of Hela cells (green) using NBP2-67353. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunocytochemistry-Immunofluorescence-NBP2-67353-img0008.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-elF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0013.jpg)
![Flow Cytometry: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Flow-Cytometry-NBP2-67353-img0001.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HUVEC cell lysateLane 2: Hela cell lysateLane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0009.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0004.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0006.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-eIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0010.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-eIF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0012.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Western-Blot-NB100-81896-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Immunohistochemistry-Paraffin-NB100-81896-img0011.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1: 1000,](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-23420241523258.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81896(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-103202523464519.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using NB100-81896](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-1132025155593.jpg)
![Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Western-Blot-NBP2-26296-img0003.jpg "Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7B11) [NBP2-26296] - eIF2A antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Immunocytochemistry-Immunofluorescence-NBP2-26296-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (3A7B11) [NBP2-26296] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast cancer using eIF2A (3A7B11) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and perinuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Immunohistochemistry-Paraffin-NBP2-26296-img0005.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0004.jpg "Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7A8) [NBP2-37279] - Immunofluorescence analysis of HepG2 cells using EIF2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Immunocytochemistry-Immunofluorescence-NBP2-37279-img0005.jpg)
![Immunohistochemistry: eIF2A Antibody (3A7A8) [NBP2-37279] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Immunohistochemistry-NBP2-37279-img0007.jpg)
![Flow Cytometry: eIF2A Antibody (3A7A8) [NBP2-37279] - Flow cytometric analysis of HepG2 cells using EIF2A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Flow-Cytometry-NBP2-37279-img0006.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mAb against human EIF2A (AA: 448-576) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0002.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mAb against HEK293 (1) and EIF2A (AA: 448-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0003.jpg)
![ELISA: eIF2A Antibody (3A7A8) [NBP2-37279] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-ELISA-NBP2-37279-img0001.jpg)
![Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of various cell lines, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Western-Blot-NBP3-16864-img0004.jpg "Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of various cell lines, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] - Immunohistochemistry of paraffin-embedded mouse testis using eIF2A Rabbit mAb (NBP3-16864) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Immunohistochemistry-Paraffin-NBP3-16864-img0003.jpg)
![Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of Rat lung, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Western-Blot-NBP3-16864-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] - Immunohistochemistry of paraffin-embedded rat pancreas using eIF2A Rabbit mAb (NBP3-16864) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Immunohistochemistry-Paraffin-NBP3-16864-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] -Analysis of paraffin-embedded Human tonsil tissue using eIF2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16864_rabbit-eif2a-mab-8v8h7-immunohistochemistry-paraffin-73202594938..png)
![Western Blot: eIF2A Antibody [NBP1-84879] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize/eIF2A-Antibody-Western-Blot-NBP1-84879-img0008.jpg "Western Blot: eIF2A Antibody [NBP1-84879] - Analysis in human cell line RPMI-8226.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody [NBP1-84879] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84879-img0007.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody [NBP1-84879] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/eIF2A-Antibody-Immunohistochemistry-Paraffin-NBP1-84879-img0002.jpg)
![Western Blot: eIF2A Antibody [NBP1-84879] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-179](http://images.novusbio.com/fullsize/eIF2A-Antibody-Western-Blot-NBP1-84879-img0006.jpg)
![Western Blot: eIF2A Antibody (S08-8E2) [NBP3-14927] - Western blot detection of eIF2A in LnCap, K562, U251MG cell lysates using eIF2A Rabbit mAb (1:500 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.](http://images.novusbio.com/fullsize/eIF2A-Antibody-S08-8E2-Western-Blot-NBP3-14927-img0001.jpg "Western Blot: eIF2A Antibody (S08-8E2) [NBP3-14927] - Western blot detection of eIF2A in LnCap, K562, U251MG cell lysates using eIF2A Rabbit mAb (1:500 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody (S08-8E2) [NBP3-14927] - Immunofluorescence of eIF2A (green) in MCF-7 using eIF2A antibody at dilution 1/20, and DAPI (blue)](http://images.novusbio.com/fullsize/eIF2A-Antibody-S08-8E2-Immunocytochemistry-Immunofluorescence-NBP3-14927-img0002.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (0011) [NBP3-23508] - Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using NBP3-23508 at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-23508_rabbit-eif2a-mab-0011-13620242234507.png "Western Blot: eIF2A [p Ser51] Antibody (0011) [NBP3-23508] - Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using NBP3-23508 at 1:1000 dilution.")