DNAM-1/CD226 Antibodies

FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="Human peripheral blood mononuclear cells (PBMCs) gated on CD3^-cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

666-DN) to Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (AVI9174). The Neutralization Dose (ND₅₀) for this effect is typically 0.300-6.00 µg/mL." class="big_lightbox"> 666-DN) to Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (AVI9174). The Neutralization Dose (ND₅₀) for this effect is typically 0.300-6.00 µg/mL." class="big_thumb" />

666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DNAM-1/ CD226 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF666). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_lightbox"> 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DNAM-1/ CD226 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF666). The ND₅₀ is typically 0.5-1.5 µg/mL." alt="Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DNAM-1/ CD226 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF666). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_thumb" />

FAB116A) and either (A) Rat Anti-Mouse DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB4436) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_lightbox"> FAB116A) and either (A) Rat Anti-Mouse DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB4436) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="Mouse splenocytes were stained with Rat Anti-Mouse CD8a APC-conjugated Monoclonal Antibody (Catalog # FAB116A) and either (A) Rat Anti-Mouse DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB4436) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="DNAM-1/CD226 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB6661) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="DNAM-1/CD226 was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human DNAM-1/CD226
Monoclonal Antibody (Catalog # MAB108921) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="DNAM-1/CD226 was detected in immersion fixed human NK cells (positive staining) and U937 human histiocytic lymphoma cell line (negative staining) using Rabbit Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB10892) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

FAB24086P) and either (A) Rabbit Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB10988) or (B) Rabbit IgG Isotype Control (MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). " class="big_lightbox"> FAB24086P) and either (A) Rabbit Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB10988) or (B) Rabbit IgG Isotype Control (MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). " alt="Human peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Rabbit Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB24086P) and either (A) Rabbit Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB10988) or (B) Rabbit IgG Isotype Control (MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). " class="big_thumb" />