Digoxigenin Antibodies
We offer Digoxigenin Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In-situ Hybridization, Southern Blot, Western Blot. Each Digoxigenin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Digoxigenin Antibodies can be used in a variety of model species: Multi-Species. Use the list below to choose the Digoxigenin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Digoxigenin polyclonal antibodies and browse our Digoxigenin monoclonal antibody catalog.
Alternate Names for Digoxigenin Antibodies
anti-Digoxigenin antibody, anti-DIG antibody
9 Results for "digoxigenin" in Primary Antibodies
![Western Blot: Digoxigenin Antibody (PSH04-39) [NBP3-32266] - Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (NBP3-32266) at 1/2,000 dilution. Lane 1: DIG-BSA (2 ng/Lane) Lane 2: BSA (negative) (2 ng/Lane) Lane 3: 293T cell lysate (negative) (20 ug/Lane) Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32266_rabbit-digoxigenin-mab-psh04-39-278202415295532.jpg "Western Blot: Digoxigenin Antibody (PSH04-39) [NBP3-32266] - Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (NBP3-32266) at 1/2,000 dilution. Lane 1: DIG-BSA (2 ng/Lane) Lane 2: BSA (negative) (2 ng/Lane) Lane 3: 293T cell lysate (negative) (20 ug/Lane) Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Digoxigenin Antibody (PSH04-39) [NBP3-32266] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxigenin antibody (NBP3-32266) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32266) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32266_rabbit-digoxigenin-mab-psh04-39-298202414304180.jpg)
![Western Blot: Digoxigenin Antibody (DIG45) - VHH [NBP3-06992] - Western Blot of Digoxigenin Antibody (DIG45). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: ~80kDa in DIG+BSA (arrow); secondary background seen in current tested lot of HEK293T Lysate.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG45-VHH-Western-Blot-NBP3-06992-img0002.jpg "Western Blot: Digoxigenin Antibody (DIG45) - VHH [NBP3-06992] - Western Blot of Digoxigenin Antibody (DIG45). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: ~80kDa in DIG+BSA (arrow); secondary background seen in current tested lot of HEK293T Lysate.")
![SDS-Page: Digoxigenin Antibody (DIG45) - VHH [NBP3-06992] - SDS-PAGE Results of Recombinant Digoxigenin Antibody (DIG45). Lane 1: Anti-DIG-VhH (Clone DIG45) Reduced (1ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Anti-DIG-VhH (Clone DIG45) Non-Reduced (1ug). 4-20% Gel Coomassie Blue stained. Observed MW: ~16kDa.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG45-VHH-SDS-Page-NBP3-06992-img0001.jpg)
![ELISA: Digoxigenin Antibody (DIG45) - VHH [NBP3-06992] - ELISA Results of purified recombinant Digoxigenin Antibody (DIG45) reactive against BSA-conjugated DIG. The working dilution for clone DIG45 is 1:2000. Each well was coated in duplicate with 1.0ug of BSA conjugated DIG (Blue) and BSA only (Purple). The starting dilution of antibody was 45ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Sodium Carbonate blocking buffer, Mouse Anti-6X HIS Epitope Tag HRP conjugated Antibody and TMB substrate.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG45-VHH-ELISA-NBP3-06992-img0003.jpg)
![Western Blot: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - Western Blot of Digoxigenin Antibody (DIG44). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Left Blot - Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Right Blot - No Primary. Secondary Only Control. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: (arrow) ~80kDa in DIG+BSA; secondary background seen in current tested lot of HEK293T Lysate.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG44-VHH-Western-Blot-NBP3-06994-img0002.jpg "Western Blot: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - Western Blot of Digoxigenin Antibody (DIG44). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Left Blot - Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Right Blot - No Primary. Secondary Only Control. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: (arrow) ~80kDa in DIG+BSA; secondary background seen in current tested lot of HEK293T Lysate.")
![SDS-Page: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - SDS-PAGE Results of Recombinant . Lane 1: Anti-DIG-VhH (Clone DIG44) Reduced (1ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Anti-DIG-VhH (Clone DIG44) Non-Reduced (1ug). 4-20% Gel Coomassie Blue stained. Predicted MW: ~15kDa. Observed MW: ~18kDa Reduced, ~16kDa Non-Reduced.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG44-VHH-SDS-Page-NBP3-06994-img0001.jpg)
![ELISA: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - ELISA Results of purified recombinant Digoxigenin Antibody (DIG44) reactive against BSA-conjugated DIG. The working dilution for clone DIG44 is 1:1000. Each well was coated in duplicate with 1.0ug BSA-conjugated DIG (Red) and BSA only (Green). The starting dilution of antibody was 45ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Sodium Carbonate blocking buffer, Mouse Anti-6X HIS Epitope Tag HRP conjugated Antibody and TMB substrate.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG44-VHH-ELISA-NBP3-06994-img0004.jpg)
