| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC7520G | |||
| Carrier Free | APM7520 | |||
| Carrier Free | HAM7520 | |||
| Carrier Free | MAB7520-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10386-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB75201-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65495-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7520R-SP | |||
| Carrier Free | MAB7520R-500 | |||
| Carrier Free | MAB7520R-01M | |||
![Western Blot: Digoxigenin Antibody (PSH04-39) [NBP3-32266] - Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (NBP3-32266) at 1/2,000 dilution. Lane 1: DIG-BSA (2 ng/Lane) Lane 2: BSA (negative) (2 ng/Lane) Lane 3: 293T cell lysate (negative) (20 ug/Lane) Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32266_rabbit-digoxigenin-mab-psh04-39-278202415295532.jpg "Western Blot: Digoxigenin Antibody (PSH04-39) [NBP3-32266] - Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (NBP3-32266) at 1/2,000 dilution. Lane 1: DIG-BSA (2 ng/Lane) Lane 2: BSA (negative) (2 ng/Lane) Lane 3: 293T cell lysate (negative) (20 ug/Lane) Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Digoxigenin Antibody (PSH04-39) [NBP3-32266] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxigenin antibody (NBP3-32266) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32266) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32266_rabbit-digoxigenin-mab-psh04-39-298202414304180.jpg)
![Western Blot: Digoxigenin Antibody (DIG45) - VHH [NBP3-06992] - Western Blot of Digoxigenin Antibody (DIG45). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: ~80kDa in DIG+BSA (arrow); secondary background seen in current tested lot of HEK293T Lysate.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG45-VHH-Western-Blot-NBP3-06992-img0002.jpg "Western Blot: Digoxigenin Antibody (DIG45) - VHH [NBP3-06992] - Western Blot of Digoxigenin Antibody (DIG45). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: ~80kDa in DIG+BSA (arrow); secondary background seen in current tested lot of HEK293T Lysate.")
![SDS-Page: Digoxigenin Antibody (DIG45) - VHH [NBP3-06992] - SDS-PAGE Results of Recombinant Digoxigenin Antibody (DIG45). Lane 1: Anti-DIG-VhH (Clone DIG45) Reduced (1ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Anti-DIG-VhH (Clone DIG45) Non-Reduced (1ug). 4-20% Gel Coomassie Blue stained. Observed MW: ~16kDa.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG45-VHH-SDS-Page-NBP3-06992-img0001.jpg)
![ELISA: Digoxigenin Antibody (DIG45) - VHH [NBP3-06992] - ELISA Results of purified recombinant Digoxigenin Antibody (DIG45) reactive against BSA-conjugated DIG. The working dilution for clone DIG45 is 1:2000. Each well was coated in duplicate with 1.0ug of BSA conjugated DIG (Blue) and BSA only (Purple). The starting dilution of antibody was 45ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Sodium Carbonate blocking buffer, Mouse Anti-6X HIS Epitope Tag HRP conjugated Antibody and TMB substrate.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG45-VHH-ELISA-NBP3-06992-img0003.jpg)
![Western Blot: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - Western Blot of Digoxigenin Antibody (DIG44). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Left Blot - Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Right Blot - No Primary. Secondary Only Control. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: (arrow) ~80kDa in DIG+BSA; secondary background seen in current tested lot of HEK293T Lysate.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG44-VHH-Western-Blot-NBP3-06994-img0002.jpg "Western Blot: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - Western Blot of Digoxigenin Antibody (DIG44). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Left Blot - Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Right Blot - No Primary. Secondary Only Control. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: (arrow) ~80kDa in DIG+BSA; secondary background seen in current tested lot of HEK293T Lysate.")
![SDS-Page: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - SDS-PAGE Results of Recombinant . Lane 1: Anti-DIG-VhH (Clone DIG44) Reduced (1ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Anti-DIG-VhH (Clone DIG44) Non-Reduced (1ug). 4-20% Gel Coomassie Blue stained. Predicted MW: ~15kDa. Observed MW: ~18kDa Reduced, ~16kDa Non-Reduced.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG44-VHH-SDS-Page-NBP3-06994-img0001.jpg)
![ELISA: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - ELISA Results of purified recombinant Digoxigenin Antibody (DIG44) reactive against BSA-conjugated DIG. The working dilution for clone DIG44 is 1:1000. Each well was coated in duplicate with 1.0ug BSA-conjugated DIG (Red) and BSA only (Green). The starting dilution of antibody was 45ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Sodium Carbonate blocking buffer, Mouse Anti-6X HIS Epitope Tag HRP conjugated Antibody and TMB substrate.](http://images.novusbio.com/fullsize/Digoxigenin-Antibody-DIG44-VHH-ELISA-NBP3-06994-img0004.jpg)
