Digoxigenin Antibody

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Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
5.0 mg/ml

Order Details

Digoxigenin Antibody Summary

Immunogen
Digoxin-3-CM conjugated to keyhole limpet haemocyanin
Specificity
Recognizes Digoxigenin, a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production. This cross reacts with Digoxin and Digitoxin. Negligible reactivity has been observed with Ouabain.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:5000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Chemical

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
5.0 mg/ml
Purity
Protein G purified

Alternate Names for Digoxigenin Antibody

  • DIG
  • Digoxigenin

Background

Digoxigenin is a small organic molecule rather than amino acids. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Digoxigenin Products

Digoxigenin NB100-65495

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