Digoxigenin Antibody (2577B) [Unconjugated]

Digoxigenin was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Digoxigenin Monoclonal Antibody (Catalog # MAB10386) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Digoxigenin labeled anti-Vimentin antibodies bound in the bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Digoxigenin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Digoxigenin Monoclonal Antibody (Catalog # MAB10386) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm incubated with Digoxigenin-labeled anti-Vimentin antibody. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 2577B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Digoxigenin Antibody (2577B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: KLH-coupled Digoxigenin
• Specificity: Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Digoxigenin Antibody (2577B) [Unconjugated]

• DIG
• Digoxigenin

Background

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.