Digoxigenin Antibody (DIG44) - VHH - BSA Free

Western Blot: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - Western Blot of Digoxigenin Antibody (DIG44). Lane 1: Opal Prestained Molecular Weight Marker (5uL). Lane 2: DIG-BSA conjugated (0.2ug). Lane 3: BSA only (0.2ug). Lane 4: HEK293T lysate (20ug). Left Blot - Primary Antibody: Anti-DIG (VhH) at 1ug/mL incubated overnight at 4 degrees C. Right Blot - No Primary. Secondary Only Control. Secondary Antibody: Mouse Anti-6X HIS at 1:1000 incubated for 1hr at RT. Blocking buffer: Universal Buffer for 2hrs at RT. Expected MW: ~80kDa in DIG+BSA. Observed MW: (arrow) ~80kDa in DIG+BSA; secondary background seen in current tested lot of HEK293T Lysate.

SDS-Page: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - SDS-PAGE Results of Recombinant . Lane 1: Anti-DIG-VhH (Clone DIG44) Reduced (1ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Anti-DIG-VhH (Clone DIG44) Non-Reduced (1ug). 4-20% Gel Coomassie Blue stained. Predicted MW: ~15kDa. Observed MW: ~18kDa Reduced, ~16kDa Non-Reduced.

ELISA: Digoxigenin Antibody (DIG44) - VHH [NBP3-06994] - ELISA Results of purified recombinant Digoxigenin Antibody (DIG44) reactive against BSA-conjugated DIG. The working dilution for clone DIG44 is 1:1000. Each well was coated in duplicate with 1.0ug BSA-conjugated DIG (Red) and BSA only (Green). The starting dilution of antibody was 45ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Sodium Carbonate blocking buffer, Mouse Anti-6X HIS Epitope Tag HRP conjugated Antibody and TMB substrate.

• Reactivity: Pl
• Applications: WB, ELISA, PAGE
• Clone: DIG44
• Clonality: Monoclonal
• Host: Llama
• Conjugate: Unconjugated
• Format: BSA Free

Digoxigenin Antibody (DIG44) - VHH - BSA Free Summary

• Description: Digoxigenin Antibody (DIG44) is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture.
  
  Store at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture
• Immunogen: Digoxigenin
• Specificity: Expect reactivity against DIG-labeled probes and proteins.
• Clonality: Monoclonal
• Host: Llama
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • SDS-Page
  • Western Blot 1:1000
• Application Notes: This product is a his-tagged monoclonal recombinant antibody designed to detect digoxigenin and its conjugates. This antibody has been tested by ELISA, SDS-PAGE, and western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-DIG can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of DIG-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user.

Reactivity Notes

Digitalis sp.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 1X PBS, pH 7.4, 20% (v/v) Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Digoxigenin Antibody (DIG44) - VHH - BSA Free

• DIG
• Digoxigenin

Background

Digoxigenin is a small organic molecule rather than amino acids. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.