CNTF Antibodies

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="CNTF was detected in perfusion fixed frozen sections of mouse cerebellum using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm of Purkinje cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm of Purkinje cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

557-NT) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA). The ND₅₀ is typ-ically 0.3-2.4 µg/mL." class="big_lightbox"> 557-NT) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA). The ND₅₀ is typ-ically 0.3-2.4 µg/mL." class="big_thumb" />

557-NT) was serially diluted and captured by Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 557-NT) was serially diluted and captured by Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glial cells in the white matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glial cells in the white matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="CNTF was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown); (CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glial cells in the white matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

557-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Rat CNTF (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA). The ND₅₀ is typically 7‑15 µg/mL." class="big_lightbox"> 557-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Rat CNTF (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA). The ND₅₀ is typically 7‑15 µg/mL." class="big_thumb" />

257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA). The ND₅₀ is typically 3-8 µg/mL." class="big_lightbox"> 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA). The ND₅₀ is typically 3-8 µg/mL." alt="Recombinant Human CNTF (Catalog # 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA). The ND₅₀ is typically 3-8 µg/mL." class="big_thumb" />

257-NT) was serially diluted and captured by Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 257-NT) was serially diluted and captured by Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

557-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557). The ND₅₀ is typically 1-3 µg/mL." class="big_lightbox"> 557-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557). The ND₅₀ is typically 1-3 µg/mL." alt="Recombinant Rat CNTF (Catalog # 557-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557). The ND₅₀ is typically 1-3 µg/mL." class="big_thumb" />

557-NT) was serially diluted and captured by Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 557-NT) was serially diluted and captured by Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657). The ND₅₀ is typically 2-6 µg/mL." class="big_lightbox"> 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657). The ND₅₀ is typically 2-6 µg/mL." alt="Recombinant Human CNTF (Catalog # 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657). The ND₅₀ is typically 2-6 µg/mL." class="big_thumb" />

257-NT) was serially diluted and captured by Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 257-NT) was serially diluted and captured by Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

257-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Human CNTF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Polyclonal Antibody (Catalog # AB-257-NA). The ND₅₀ is typically 15-30 µg/mL." class="big_lightbox"> 257-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Human CNTF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Polyclonal Antibody (Catalog # AB-257-NA). The ND₅₀ is typically 15-30 µg/mL." alt="Recombinant Human CNTF (Catalog # 257-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Human CNTF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Polyclonal Antibody (Catalog # AB-257-NA). The ND₅₀ is typically 15-30 µg/mL." class="big_thumb" />