CLEC9a Antibodies

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="CLEC9a was detected in perfusion fixed frozen sections of mouse spleen using Sheep Anti-Mouse CLEC9a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6776) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dendritic cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF016). A specific band was detected for CLEC9a at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for CLEC9a at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human CD14⁺peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CLEC9a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6049) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CLEC9a at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Anti-Mouse Integrin alpha X/CD11c Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB69051R). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Anti-Mouse Integrin alpha X/CD11c Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB69051R). View our protocol for Staining Membrane-associated Proteins." alt="Mouse splenocytes were stained with (A) Rabbit Anti-Mouse CLEC9a Monoclonal Antibody (Catalog # MAB67762) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Anti-Mouse Integrin alpha X/CD11c Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB69051R). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />