CKII alpha prime polypeptide Antibodies
We offer CKII alpha prime polypeptide Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot. Each CKII alpha prime polypeptide Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CKII alpha prime polypeptide Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the CKII alpha prime polypeptide Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CKII alpha prime polypeptide polyclonal antibodies and browse our CKII alpha prime polypeptide monoclonal antibody catalog.
Alternate Names for CKII alpha prime polypeptide Antibodies
anti-CKII alpha prime polypeptide antibody, anti-CSNK2A2 antibody, anti-casein kinase 2, alpha prime polypeptide antibody, anti-casein kinase II subunit alpha' antibody, anti-CK II alpha' antibody, anti-CK2A2 antibody, anti-CSNK2A1 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.1 antibody, anti-FLJ43934 antibody
13 Results for "ckii-alpha-prime-polypeptide" in Primary Antibodies
![Western Blot: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of Human and Mouse CKII alpha' by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NB100-379-img0004.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of Human and Mouse CKII alpha' by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of human CKII alpha' by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for IP at 6 ug per reaction. CKII alpha' was also immunoprecipitated by rabbit anti-CKII alpha' antibody NB100-378. For blotting immunoprecipitated CKII alpha', NB100-379 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Immunoprecipitation-NB100-379-img0005.jpg)
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35347] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241512133.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35347] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202414213764.jpg)
![Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunofluorescence analysis of NIH-3T3 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202415171197.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-912202414163074.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241418158.jpg)
![Immunohistochemistry: CKII alpha prime polypeptide Antibody [NBP3-35347] - Immunohistochemistry analysis of paraffin-embedded Rat lung using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35347_rabbit-ckii-alpha-prime-polypeptide-pab-91220241514367.jpg)
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35650_rabbit-ckii-alpha-prime-polypeptide-pab-10122024120692.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] - Western blot analysis of lysates from HeLa cells, using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35650_rabbit-ckii-alpha-prime-polypeptide-pab-1012202411572658.jpg)
![Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35650] - Immunofluorescence analysis of NIH/3T3 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35650_rabbit-ckii-alpha-prime-polypeptide-pab-101220241214447.jpg)
![Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35650] - Immunofluorescence analysis of PC-12 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35650_rabbit-ckii-alpha-prime-polypeptide-pab-1012202411572640.jpg)
![Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686] - Anti-CKII alpha prime polypeptide rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NBP2-99686-img0005.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686] - Anti-CKII alpha prime polypeptide rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-99686] - Immunofluorescence staining of CKII alpha prime polypeptide in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CKII alpha prime polypeptide polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99686-img0002.jpg)
![Immunoprecipitation: CKII alpha prime polypeptide Antibody [NBP2-99686] - CKII alpha prime polypeptide was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate4 ul anti-CKII alpha prime polypeptide rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CKII alpha prime polypeptide rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Immunoprecipitation-NBP2-99686-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody (1E8) [H00001459-M03] - Analysis of monoclonal antibody to CSNK2A2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-1E8-Immunocytochemistry-Immunofluorescence-H00001459-M03-img0002.jpg "Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody (1E8) [H00001459-M03] - Analysis of monoclonal antibody to CSNK2A2 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: CKII alpha prime polypeptide Antibody (1E8) [H00001459-M03] - Detection limit for recombinant GST tagged CSNK2A2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-1E8-Sandwich-ELISA-H00001459-M03-img0003.jpg)
![Western Blot: CKII alpha prime polypeptide Antibody (3W5P4) [NBP3-16076] - Western blot analysis of various lysates, using CKII alpha prime polypeptide Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16076_rabbit-ckii-alpha-prime-polypeptide-mab-3w5p4-7220251245330.jpg "Western Blot: CKII alpha prime polypeptide Antibody (3W5P4) [NBP3-16076] - Western blot analysis of various lysates, using CKII alpha prime polypeptide Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-57977] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57977-img0001.jpg "Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-57977] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP1-56402] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NBP1-56402-img0002.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP1-56402] - HepG2 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP1-56690] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NBP1-56690-img0002.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP1-56690] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP3-37929] - Western blot analysis of various lysates, using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-37929_rabbit-ckii-alpha-prime-polypeptide-pab-101220241233818.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP3-37929] - Western blot analysis of various lysates, using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody (4F2) [H00001459-M01] - Analysis of monoclonal antibody to CSNK2A2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-4F2-Immunocytochemistry-Immunofluorescence-H00001459-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody (4F2) [H00001459-M01] - Analysis of monoclonal antibody to CSNK2A2 on HeLa cell . Antibody concentration 10 ug/ml.")