CD68/SR-D1 Antibodies
Human CD68, also known as Scavenger Receptor D1 (SR-D1), is a transmembrane glycoprotein that belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. CD68/SR-D1 has a theoretical molecular weight of 110 kDa and is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells. We offer antibodies to detect CD68/SR-D1 that are highly reviewed and have been cited in 110+ publications. Browse our extensive catalog of recombinant monoclonal antibodies, monoclonal antibodies, polyclonal antibodies, and CD68/SR-D1-conjugated antibodies. Our CD68/SR-D1 antibodies are suitable for multiple validated research applications including Chromatin Immunoprecipitation, CyTOF-ready, Dual RNAscope ISH-IHC, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Paraffin, Immunoprecipitation, and Knockdown Validated Western Blot. Our CD68/SR-D1 antibodies can be used in a variety of model species including Human, Mouse, Rat, Bovine, Feline, and Primate. Each CD68/SR-D1 antibody is fully covered by Novus’ Guarantee+. Choose the anti-CD68/SR-D1 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for CD68/SR-D1 Antibodies
anti-CD68/SR-D1 antibody, anti-CD68 antibody, anti-CD68 antigenmacrophage antigen CD68 antibody, anti-CD68 molecule antibody, anti-DKFZp686M18236 antibody, anti-GP110 antibody, anti-macrosialin antibody, anti-SCARD1 antibody, anti-scavenger receptor class D, member 1 antibody
39 Results for "Cd68-sr-d1" in Primary Antibodies
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) [NBP2-46168] - CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human lymph node tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0010.jpg "Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) [NBP2-46168] - CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human lymph node tissue.")
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) [NBP2-46168] - CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0011.jpg)
![Western Blot: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD68.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Western-Blot-NBP2-46168-img0013.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0012.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0001.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0002.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0003.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0004.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0005.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0006.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0007.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0008.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (1E6) [NBP2-46168] - Analysis of Human bladder tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-1E6-Immunohistochemistry-NBP2-46168-img0009.jpg)
![Western Blot: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD68.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Western-Blot-NBP2-70381-img0001.jpg "Western Blot: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD68.")
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0012.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0002.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0003.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0004.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0005.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0006.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0007.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Human bladder tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0008.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0009.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0010.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Human lymph node tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0011.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-OTI1E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70381-img0013.jpg)
![Western Blot: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Western Blot Analysis of human Spleen tissue lysate using CD68/SR-D1 Antibody (LAMP4/824).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-LAMP4-824-Western-Blot-NBP2-44543-img0005.jpg "Western Blot: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Western Blot Analysis of human Spleen tissue lysate using CD68/SR-D1 Antibody (LAMP4/824).")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD68/SR-D1 Antibody (LAMP4/824).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-LAMP4-824-Immunohistochemistry-Paraffin-NBP2-44543-img0004.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Flow Cytometric Analysis of U87MG cells using CD68/SR-D1 Antibody (LAMP4/824) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-LAMP4-824-Flow-Cytometry-NBP2-44543-img0006.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (LAMP4/824) [NBP2-44543] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68/SR-D1 Antibody (LAMP4/824).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-LAMP4-824-Immunohistochemistry-Paraffin-NBP2-44543-img0003.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-278202415295599.jpg "Western Blot: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32163) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-298202414115445.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32163) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-29202410234233.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Flow cytometric analysis of THP-1 (positive) and Jurkat (negative) cells labeling CD68/SR-D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32163, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-392024884626.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1 + C68/684) [NBP2-34329] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD68/SR-D1 Antibody (KP1 + C68/684).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-+-C68-684-Immunohistochemistry-Paraffin-NBP2-34329-img0008.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1 + C68/684) [NBP2-34329] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD68/SR-D1 Antibody (KP1 + C68/684).")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1 + C68/684) [NBP2-34329] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68/SR-D1 Antibody (KP1 + C68/684).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-KP1-+-C68-684-Immunohistochemistry-Paraffin-NBP2-34329-img0007.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32162) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29820241435295.jpg "Immunohistochemistry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32162) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Immunocytochemistry analysis of THP-1 cells labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32162) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32162) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29202410334075.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (PDM0-13) [NBP3-32162] - Flow cytometric analysis of THP-1 cells labeling CD68/SR-D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32162, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32162_mouse-cd68-sr-d1-mab-pdm0-13-29202416453272.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 1 minute 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-278202415212467.jpg "Western Blot: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 1 minute 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32164) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-298202414161818.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32164) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-29202410234268.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32165) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-278202415295560.jpg "Western Blot: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32165) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32165) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-29820241433363.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C4-R) [NBP3-32165] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32165) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32165) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32165_mouse-cd68-sr-d1-mab-a3c4-r-2920241048480.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1338) [NBP2-34481] - Staining of human liver shows strong immunoreactivity in Kupffer cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1338-Immunohistochemistry-NBP2-34481-img0001.jpg "Immunohistochemistry: CD68/SR-D1 Antibody (CL1338) [NBP2-34481] - Staining of human liver shows strong immunoreactivity in Kupffer cells.")
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1338) [NBP2-34481] - Staining of human cerebellum shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1338-Immunohistochemistry-NBP2-34481-img0004.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1338) [NBP2-34481] - Staining of human colon shows strong positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1338-Immunohistochemistry-NBP2-34481-img0002.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1338) [NBP2-34481] - Staining of human tonsil shows strong immunoreactivity in single cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1338-Immunohistochemistry-NBP2-34481-img0003.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (Y1/82A) [NBP3-09124] - Separation of human monocytes (red-filled) from CD68 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD68 (Y1/82A) purified antibody (concentration in sample 2 ug/ml) GAM FITC.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-Y1-82A-Flow-Cytometry-NBP3-09124-img0002.jpg "Flow Cytometry: CD68/SR-D1 Antibody (Y1/82A) [NBP3-09124] - Separation of human monocytes (red-filled) from CD68 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD68 (Y1/82A) purified antibody (concentration in sample 2 ug/ml) GAM FITC.")
![Flow Cytometry: CD68/SR-D1 Antibody (Y1/82A) [NBP3-09124] - Intracellular staining pattern of human peripheral blood stained using anti-human CD68 (Y1/82A) purified antibody (concentration in sample 2 ug/ml) GAM FITC.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-Y1-82A-Flow-Cytometry-NBP3-09124-img0001.jpg)