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![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0018.jpg "Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.")
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0021.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (NB110-96877) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0016.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug).. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 2 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0017.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0014.jpg)
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immuno-peroxidase (DAB) staining for nitrotyrosine (upper row) and immuno-alkaline phosphatase (ALP) staining for 8-OHdG (bottom row), hematoxylin counterstain. Representative small intestinal tumor histopathology of Category 1 (low-grade adenoma) in ApcMin/+ (left column) and ApcMin/+ Mieap-/- (second from left column) mice, Category 2 (high-grade adenoma, second from right column) and Category 3 (adenocarcinoma, right column) in the ApcMin/+ Mieap-/- mice. Scale bars: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12472), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-Paraffin-NB110-96877-img0015.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0019.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0020.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0022.jpg)
![Western Blot: Nitrotyrosine Antibody (2A12) [NBP3-30876] - A. Western blot using Novus Biologicals' anti 3-nitrotyrosine monoclonal antibody (Cat # NBP3-30876) on 40 ug mouse brain lysate (Lane 1) and 40 ug rat brain lysate (Lane 2). Antibody used at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate-30 second exposure.B. Same experiment blocked with buffer containing 1 mM 3-nitrosine.](http://images.novusbio.com/fullsize/nbp3-30876_mouse-nitrotyrosine-mab-2a12-811202420592158.jpg "Western Blot: Nitrotyrosine Antibody (2A12) [NBP3-30876] - A. Western blot using Novus Biologicals' anti 3-nitrotyrosine monoclonal antibody (Cat # NBP3-30876) on 40 ug mouse brain lysate (Lane 1) and 40 ug rat brain lysate (Lane 2). Antibody used at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate-30 second exposure.B. Same experiment blocked with buffer containing 1 mM 3-nitrosine.")
![ELISA: 3-Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-66363] - Samples were spiked with high concentrations of 3-Nitrotyrosine and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66363_3-nitrotyrosine-elisa-kit-colorimetric-132202416211333.jpg "ELISA: 3-Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-66363] - Samples were spiked with high concentrations of 3-Nitrotyrosine and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: 3-Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-66363] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66363_3-nitrotyrosine-elisa-kit-colorimetric-4320249141621.jpg)