3-Nitrotyrosine ELISA Kit (Colorimetric)


Universal 3-Nitrotyrosine ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
1.56 - 100 ng/mL
0.94 ng/mL

Order Details

3-Nitrotyrosine ELISA Kit (Colorimetric) Summary

This kit recognizes 3-NT in samples. No significant cross-reactivity or interference between 3-NT and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
0.94 ng/mL
Assay Type
CV% < 5.22%
CV% < 5.43%
Spike Recovery
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)



Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent


Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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