Nitrotyrosine Antibody (2A12) - BSA Free

Western Blot: Nitrotyrosine Antibody (2A12) [NBP3-30876] - A. Western blot using Novus Biologicals' anti 3-nitrotyrosine monoclonal antibody (Cat # NBP3-30876) on 40 ug mouse brain lysate (Lane 1) and 40 ug rat brain lysate (Lane 2). Antibody used at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate-30 second exposure.B. Same experiment blocked with buffer containing 1 mM 3-nitrosine.

• Reactivity: All-NA
• Applications: WB
• Clone: 2A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Nitrotyrosine Antibody (2A12) - BSA Free Summary

• Immunogen: 3-Nitrotyrosine-KLH
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot : 1:1000
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5
• Preservative: 3mM Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Nitrotyrosine Antibody (2A12) - BSA Free

• Nitrotyrosine

Background

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.