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HAF017). Specific bands were detected for Osteoactivin/GPNMB at approximately 95 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
2550-AC) was serially diluted and captured by Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
2550-AC) was serially diluted and captured by Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4-10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/ GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND50 is typically 0.2-0.8 µg/mL." class="big_lightbox" target="_blank">
2330-AC) was serially diluted and captured by Goat Anti-Mouse Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
2330-AC) was serially diluted and captured by Goat Anti-Mouse Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
The GPNMB gene codes for a type I transmembrane glycoprotein NBM. The GPNMB gene is present in lowly, but not highly, metastic human melanoma cell lines, therefore, it demonstrates involvement in development, proliferation, and differentiation of tissues. Isoform 1 is 572 amino acids long at a mass of nearly 64 kDA and isoform 2 exists at 560 amino acids long at approximately 62 kDA. This gene has been known to interact with various genes such as SMAD4, PLA2G4A, and ITSN2. GPNMB has been researched in its role in retinitis, lung cancer, melanoma, glioblastoma, glaucoma, and breast cancer.
![Western Blot Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Osteoactivin_AF2550_Western_Blot_19926.jpg)
![Neutralization Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Osteoactivin_AF2330_Block_Neutralize_574.jpg)
![N/A Osteoactivin/GPNMB [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Osteoactivin_DY2550_ELISA_1844.jpg)
![N/A Osteoactivin/GPNMB [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Osteoactivin_DY2330_ELISA_1813.jpg)
![SDS-Page Osteoactivin/GPNMB [Unconjugated]](http://images.novusbio.com/fullsize/11154-ac_recombinant-cyno-rhesus-osteoactivin-gpnmb-fc-protein-cf-sds-page-18102022113358.jpg)
![Immunohistochemistry Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Osteoactivin_AF2550_Immunohistochemistry_15337.jpg)
![Knockdown Validated Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-61220241239347.png)
![Knockdown Validated Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-61220241239340.png)
![Immunoprecipitation Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/antibody/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-immunoprecipitation-612025141810.png)
![Immunohistochemistry Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-8120255552895.jpg)
![Immunohistochemistry Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-8120255552883.jpg)
![Immunohistochemistry Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-8120255531670.jpg)
![Western Blot Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-western-blot-12122025917202.jpg)
![Western Blot Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-western-blot-12122025914024.jpg)
![Western Blot Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-western-blot-121220258575919.jpg)
![Western Blot Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-western-blot-12122025852810.jpg)
![ELISA Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/images/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-elisa-639201776850192622.jpg)
![ELISA Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/images/af2550_human-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-elisa-639201854771411766.jpg)
![Western Blot Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2330_mouse-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-8120255485729.jpg)
![Immunohistochemistry Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2330_mouse-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-8120255541757.jpg)
![ELISA Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/images/af2330_mouse-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-elisa-639201776494760483.jpg)
![ELISA Osteoactivin/GPNMB Antibody [Unconjugated]](http://images.novusbio.com/images/af2330_mouse-osteoactivin-gpnmb-affinity-purified-polyclonal-ab-elisa-639201854771485722.jpg)
