The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating both nuclear and mitochondrial gene transcription. The complex also includes pol II, and transcription factors TFIIA-F. It serves as a central scaffold within the pre-initiation complex (PIC) responsible for converting biological stimuli (in the form of transcription factors) into gene expression responses. A good review of Mediator function and influencing factors can be found in Poss et al1.
-Immunohistochemistry-Paraffin-NBP2-31146-img0003.jpg)
Immunohistochemistry-Paraffin: NUT Antibody
Additionally, the poorly understood disease known as NUT midline carcinoma (NMC) is a rare and aggressive squamous carcinoma involving chromosomal rearrangements that generate a NUT protein fused with either one of the bromodomain-containing proteins BRD4 and BRD32. A research group from Sweden believes that the Mediator complex may have also have a different role in telomeric silencing and cell senescence and lifespan determination3. They found that Mediator directly associates with telomeric heterochromatin between active and inactive content.
Novus Biologicals offers NUT reagents for your research needs including:
PMIDs
