HAF018). A specific band was detected for TMED1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for TMED1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, HDLM-2 human Hodgkin's lymphoma cell line, RT-4 human bladder carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TMED1 Monoclonal Antibody (Catalog # MAB22431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TMED1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) TMED-1 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human TMED-1 Monoclonal Antibody (Catalog # MAB2243) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

523-ST) binds with an ED₅₀ of 0.6-3.6 µg/mL." class="big_lightbox"> 523-ST) binds with an ED₅₀ of 0.6-3.6 µg/mL." alt="When Recombinant TMED1 Fc Chimera (Catalog # 2243-TM) is coated at 0.5 µg/mL, Recombinant Human ST2/IL-1 R4 Fc Chimera (Catalog # 523-ST) binds with an ED₅₀ of 0.6-3.6 µg/mL." class="big_thumb" />