V alpha 24 J alpha 18 TCR Antibody (6B11)

Images

 
Flow Cytometry: V alpha 24 J alpha 18 TCR Antibody (6B11) [NBP2-00267] - Staining of normal human peripheral blood mononuclear cells with Anti-Human CD3 FITC and 1.0 ug of Mouse IgG1 K Isotype Control Purified (left) ...read more
Flow (Cell Surface): V alpha 24 J alpha 18 TCR Antibody (6B11) [NBP2-00267] - A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Flow-CS, ICC/IF, IHC-Fr, CyTOF-ready
Clone
6B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

V alpha 24 J alpha 18 TCR Antibody (6B11) Summary

Immunogen
a peptide epitope in the CDR3 region of V alpha 24
Marker
iNKT Cells Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • CyTOF-ready
  • Flow (Cell Surface)
Application Notes
This 6B11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID16867051). Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature
Publications
Read Publications using
NBP2-00267 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for V alpha 24 J alpha 18 TCR Antibody (6B11)

  • iNKT cell
  • iNKT
  • invariant natural killer T cell
  • invariant NK T cell
  • invariant NKT cell

Background

The 6B11 monoclonal antibody reacts with an epitope of the CDR3 formed by the germ-line configuration of the V alpha 24 and J alpha 18 of the TCR alpha locus, which results in the expression of an invariant T-cell receptor (TCR) expressed by invariant NK T cells (iNKT). This TCR is homologous to the murine V alpha 14 J alpha 18. This TCR alpha chain pairs with a restricted set of rearranged TCR beta chains, with V beta 11 being the most prominent in humans. NK T cells are restricted by the CD1d molecule and are activated by a CD1d-resented glycolipid ligand, which results in the rapid production of IL-4, IL-13 and IFN gamma. Although iNKT cells have NK-like cytolyticactivity, they are considered regulators of immune responses because they rapidly produce large amounts of both Th1 and Th2 cytokines in autoimmune disease, infectious disease, and cancer. The number of iNKT cells present in peripheral blood of normal humans shows considerable variability ranging from 0.01-1% of total peripheral T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for V alpha 24 J alpha 18 TCR Antibody (NBP2-00267)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, ICC/IF, IHC-Fr.


Filter By Application
FLOW
(10)
ICC/IF
(1)
IHC-Fr
(2)
All Applications
Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-00267 Applications Species
van der Vliet HJ, Koon HB, Yue SC et al. Effects of the administration of high-dose interleukin-2 on immunoregulatory cell subsets in patients with advanced melanoma and renal cell cancer. Clin Cancer Res. 2007 Apr 1 [PMID: 17404092] (FLOW, Human) FLOW Human
Kis J, Engelmann P, Farkas K et al. Reduced CD4+ subset and Th1 bias of the human iNKT cells in Type 1 diabetes mellitus. J Leukoc Biol. 2007 Mar [PMID: 17151140]
Akbari O. The role of iNKT cells in development of bronchial asthma: a translational approach from animal models to human. Allergy. 2006 Aug [PMID: 16867051] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
Pham-Thi N, de Blic J, Leite-de-Moraes MC. Invariant natural killer T cells in bronchial asthma. N Engl J Med. 2006 Jun 15 [PMID: 16775881] (FLOW, Human) FLOW Human
Berzins SP, Smyth MJ, Godfrey DI. Working with NKT cells--pitfalls and practicalities. Curr Opin Immunol. 2005 Aug [PMID: 15963710] (IHC-Fr, FLOW, Human) IHC-Fr, FLOW Human
Bollyky PL, Wilson SB. CD1d-restricted T-cell subsets and dendritic cell function in autoimmunity. Immunol Cell Biol. 2004 Jun [PMID: 15186262] (FLOW, Human) FLOW Human
Fuss IJ, Heller F, Boirivant M et al. Nonclassical CD1d-restricted NK T cells that produce IL-13 characterize an atypical Th2 response in ulcerative colitis. J Clin Invest. 2004 May [PMID: 15146247] (FLOW, Human) FLOW Human
Metelitsa LS, Wu HW, Wang H et al. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2. J Exp Med. 2004 May 3 [PMID: 15123743] (FLOW, IHC-Fr, Human) FLOW, IHC-Fr Human
Levy O, Orange JS, Hibberd P et al. Disseminated varicella infection due to the vaccine strain of varicella-zoster virus, in a patient with a novel deficiency in natural killer T cells. J Infect Dis. 2003 Oct 1 [PMID: 14513412] (FLOW, Human) FLOW Human
Hou R, Goloubeva O, Neuberg DS et al. Interleukin-12 and interleukin-2-induced invariant natural killer T-cell cytokine secretion and perforin expression independent of T-cell receptor activation. Immunology. 2003 Sep [PMID: 12941138] (FLOW, Human) FLOW Human
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-00267AF405
Alexa Fluor 488 Labeled NBP2-00267AF488
Alexa Fluor 647 Labeled NBP2-00267AF647
Alexa Fluor 700 Labeled NBP2-00267AF700
Allophycocyanin Labeled NBP2-00267APC
DyLight 350 Labeled NBP2-00267UV
DyLight 405 Labeled NBP2-00267V
DyLight 488 Labeled NBP2-00267G
DyLight 680 Labeled NBP2-00267FR
DyLight 755 Labeled NBP2-00267IR
PE Labeled NBP2-00267PE
PerCP Labeled NBP2-00267PCP

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