Flow Cytometry: V alpha 24 J alpha 18 TCR Antibody (6B11) [NBP2-00267] - Staining of normal human peripheral blood mononuclear cells with Anti-Human CD3 FITC and 1.0 ug of Mouse IgG1 K Isotype Control Purified (left) ...read more
Flow (Cell Surface): V alpha 24 J alpha 18 TCR Antibody (6B11) [NBP2-00267] - A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched ...read more
This 6B11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID16867051). Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature. This antibody is CyTOF ready.
Read Publications using NBP2-00267 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein G purified
Alternate Names for V alpha 24 J alpha 18 TCR Antibody (6B11)
invariant natural killer T cell
invariant NK T cell
invariant NKT cell
The 6B11 monoclonal antibody reacts with an epitope of the CDR3 formed by the germ-line configuration of the V alpha 24 and J alpha 18 of the TCR alpha locus, which results in the expression of an invariant T-cell receptor (TCR) expressed by invariant NK T cells (iNKT). This TCR is homologous to the murine V alpha 14 J alpha 18. This TCR alpha chain pairs with a restricted set of rearranged TCR beta chains, with V beta 11 being the most prominent in humans. NK T cells are restricted by the CD1d molecule and are activated by a CD1d-resented glycolipid ligand, which results in the rapid production of IL-4, IL-13 and IFN gamma. Although iNKT cells have NK-like cytolyticactivity, they are considered regulators of immune responses because they rapidly produce large amounts of both Th1 and Th2 cytokines in autoimmune disease, infectious disease, and cancer. The number of iNKT cells present in peripheral blood of normal humans shows considerable variability ranging from 0.01-1% of total peripheral T cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for V alpha 24 J alpha 18 TCR Antibody (NBP2-00267)(12)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 3 applications: FLOW, ICC/IF, IHC-Fr.
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