V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free

Flow (Cell Surface): V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free [NBP2-81003] - A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin. A co-stain was also performed using CD3 antibody NBP2-24867APC.

Flow Cytometry: V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free [NBP2-81003] - Staining of normal human peripheral blood mononuclear cells with Anti-Human CD3 FITC and 1.0 ug of Mouse IgG1 K Isotype Control Purified (left) or 1.0 ug of Anti-Human V alpha 24 J alpha 18 TCR Purified (right) followed by F(ab')2 Anti-Mouse IgG PE . CD Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 6B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free Summary

• Immunogen: Peptide epitope in the CDR3 region of V alpha 24
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 1:10-1:1000
• Application Notes: This 6B11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free

• iNKT cell
• iNKT
• invariant natural killer T cell
• invariant NK T cell
• invariant NKT cell

Background

The 6B11 monoclonal antibody reacts with an epitope of the CDR3 formed by the germ-line configuration of the V alpha 24 and J alpha 18 of the TCR alpha locus, which results in the expression of an invariant T-cell receptor (TCR) expressed by invariant NK T cells (iNKT). This TCR is homologous to the murine V alpha 14 J alpha 18. This TCR alpha chain pairs with a restricted set of rearranged TCR beta chains, with V beta 11 being the most prominent in humans. NK T cells are restricted by the CD1d molecule and are activated by a CD1d-resented glycolipid ligand, which results in the rapid production of IL-4, IL-13 and IFN gamma. Although iNKT cells have NK-like cytolyticactivity, they are considered regulators of immune responses because they rapidly produce large amounts of both Th1 and Th2 cytokines in autoimmune disease, infectious disease, and cancer. The number of iNKT cells present in peripheral blood of normal humans shows considerable variability ranging from 0.01-1% of total peripheral T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.