T Cell Receptor alpha Chain-V alpha 24-J alpha Q Antibody

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line, mouse spleen, rat and, mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat T Cell Receptor a Chain-V a24-J aQ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for T Cell Receptor a Chain-V a24-J aQ at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

T Cell Receptor alpha Chain-V alpha 24-J alpha Q Antibody Summary

• Immunogen: E. coli-derived recombinant human T Cell Receptor alpha Chain-V alpha 24-J alpha Q
  Lys22-Pro135
  Accession # ABC72391
• Specificity: Detects human, mouse, and rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for T Cell Receptor alpha Chain-V alpha 24-J alpha Q Antibody

• T Cell Receptor alpha Chain-V alpha 24-J alpha Q
• T CellReceptor alpha ChainV alpha 24J alpha Q

Background

V alpha 24-J alpha Q (T cell receptor alpha chain) is a 31 kDa (predicted) member of the Ig superfamily. It constitutes an invariant alpha -chain that is paired with the V beta 11 chain on human NKT INV cells, a subpopulation of NKR-P1A T cells. This TCR creates a binding site for CD1d-associated ligands that are presumed to involve glyco-/phospholipids. The human V alpha 24V beta 11 TCR is analogous to the V alpha 14V beta 8 complex in mice. Human V alpha 24-J alpha Q is 276 amino acids in length. It contains a 21 aa signal sequence, multiple N-linked glycosylation sites, a V-type Ig-like domain (aa 36-133) and a TCR-related sequence of unknown function (aa 142-255). Considerable variability is noted over aa 22-114. The closest mouse ortholog to human V alpha 24-J alpha Q shows 65% aa identity over aa 22-135.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • ABC72391()